血清ALT与AST测定的基质效应评价

摘要：目的：评价7种室间质量评价（EQA）材料和4种市售材料在9个检测系统上测定血清ALT和AST的基质效应。 方法：参照美国临床实验室标准化协会(CLSI)EP14-A2指南,根据ALT和AST的参考方法建立不含磷酸吡哆醛的比对方法。9个检测系统的常规方法为待评方法。用比对方法和待评方法同时测定新鲜人血清、EQA材料及市售材料的ALT和AST水平，评价非血清样品的基质效应。 结果：ALT测定中1种EQA材料对所有检测系统均表现基质效应，AST测定中2种EQA材料对所有检测系统无基质效应，其他材料视系统的不同而表现出不同的情况。 结论：基质效应影响血清ALT和AST测定的准确性和可比性，应充分重视基质效应对临床检验量值溯源的影响。

Evaluation of matrix effects for serum ALT and AST measurements

Objective To evaluate the matrix effects of measurement for alanine transaminase(ALT) and aspartate transaminase(AST) in serum by using 7 materials of external quality assessment(EQA) and 4 commercial reference materials in 9 testing systems.Methods According to the Clinical and Laboratory Standards Institute(CLSI) EP14-A2 guideline and the reference methods of ALT and AST measurement,the comparative methods without pyridoxal 5-phosphate were developed.The routine measurement methods of 9 testing systems we...