| 双抗体夹心ELISA法检测嗜酸性粒细胞阳离子蛋白 |
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| 引用本文： | 赵俊芳,王学谦,李桂珍,李会强.双抗体夹心ELISA法检测嗜酸性粒细胞阳离子蛋白[J].临床检验杂志,2011,29(5):336-338. |
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| 作者姓名： | 赵俊芳 王学谦 李桂珍 李会强 |
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| 作者单位： | 天津市长征医院检验中心，天津300120;天津医院检验科，天津300211;天津市长征医院检验中心，天津300120;天津医科大学免疫学教研室，天津300070 |
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| 摘 要： | 目的 制备嗜酸性粒细胞阳离子蛋白(eosinophilia cationic protein,ECP)双抗体夹心ELISA法检测试剂盒.方法 葡萄球菌A蛋白亲和层析法纯化抗ECP单克隆抗体,过碘酸钠法进行抗ECP多克隆抗体HRP标记,制备ELISA试剂盒,进行条件优化和实验效能评价,检测过敏性皮肤病患者ECP水平.结果...
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| 关 键 词： | 嗜酸性粒细胞阳离子蛋白 酶联免疫吸附试验 过敏性疾病 |
| 收稿时间： | 2010/9/10 0:00:00 |
| 修稿时间： | 2011/3/25 0:00:00 |
Double-antibody sandwich ELISA for determination of eosinophilia cationic protein in serum |
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| ZHAO Jun-fang,WANG Xue-qian,LI Gui-zhen,LI Hui-qiang.Double-antibody sandwich ELISA for determination of eosinophilia cationic protein in serum[J].Chinese Journal of Clinical Laboratory Science,2011,29(5):336-338. |
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| Authors: | ZHAO Jun-fang WANG Xue-qian LI Gui-zhen LI Hui-qiang |
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| Institution: | ZHAO Jun-fang1,WANG Xue-qian2,LI Gui-zhen1,LI Hui-qiang3(1.Department of Clinical Laboratory,Tianjin Changzheng Hospital,Tianjin 300120,2.Department of Laboratory Medicine,Tianjin Hospital,Tianjin 300211,3.Department of Immunology,Tianjin Medical University,Tianjin 300070,China) |
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| Abstract: | Objective To develop a double-antibody sandwich ELISA for detecting eosinophilia cationic protein(ECP).Methods Anti-ECP monoclonal antibody(mAb) was purified by affinity chromatography with staphylococcal protein A.Anti-ECP polyclonal antibody(pAb) was labeled with horseradish peroxidase by sodium periodate(NaIO4) method.The experimental condition of ELISA was optimized and the experimental performance was evaluated.The serum samples of patients with allergic dermatic diseaseswere collected and the ECP leve... |
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| Keywords: | eosinophilia cationic protein enzyme linked immunosorbent assay determination allergic diseases |
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