人参、三七提取物对Ras相关信号蛋白的影响

目的 探讨益气活血中药人参、三七提取物对血管内皮细胞血管生成信号蛋白血管内皮生长因子受体-2（VEGFR-2）、Ras及有丝分裂原活化蛋白激酶（MAPK）表达的影响。

Effect of Ginseng-Sanqi Extract on the Ras associated Signal Proteins

Objective To explore the effect of ginseng-sanqi extract（GSE） on the vascular endothelium growth factor receptor-2（VEGFR-2）,Ras and mitogen-activated protein kinase（MAPK） in VEGFR-2-Ras-MAPK signal pathway of angiogenesis in cultured human umbilical vascular endothelial cells（HUVECs）.Methods The protein expressions of VEGFR-2,Ras and MAPK in HUVEC and the effects of GSE（at different doses） and basic fibrin growth factor（bFGF） on them were detected by Western-Blot test.Results GSE can enhance the expression of angiogenesis signaling proteins（VEGFR-2,Ras,MAPK） to different extents,especially in the groups treated by bFGF or higher dose GSE,the levels showed significant differences to those in the blank group（P〈0.05）.Conclusion GSE can promote the expressions of VEGFR-2,Ras and MAPK in the angiogenesis signaling pathway,which may be the foundation of Chinese medicine for tonifying qi and activating blood circulation in promoting endothelial proliferation and angiogenesis.