乙肝病人T细胞亚群、mIL-2R的变化及意义

目的检测乙型病毒性肝炎病人外周血T细胞亚群和膜白介素-2受体(mIL—2R)表达水平并探讨其在乙肝发病机制中的作用。方法采用生物素-链霉亲和素法对188例乙肝病人外周血单个核细胞(PBMC)进行T细胞亚群及植物血凝素(PHA)诱导前后mIL—2R水平测定。结果乙肝病人T淋巴细胞亚群中CD3~+、CD4~+降低,CD8~+升高,CD4~+/CD8~+比值降低,外周血单个核细胞mIL—2R表达水平在PHA诱导前后均降低,与正常对照相比,差异有显著意义(P<0.01);另外,乙肝病人PBMC中HBV—DNA(+)病人与PBMC内HBV—DNA(-)病人相比,mIL—2R表达水平亦存在较大差异(P<0.01)。结论乙肝病人体内存在明显的细胞免疫功能紊乱,T细胞活化障碍,并与肝病的慢性化相关。

Detection and significance of T lymphocyte subsets and mIL-2R in patients with hepatitis B

Objective To study the levels of T lymphocyte subsets and membrane interleukin-2 receptor (mIL-2R) on surface of peripheral blood mononuclear cells (PBMC) of the patients with hepatitis B and its role in the pathogenesis of hepatitis B. Methods The levels of T lymphocyte subsets and mIL-2R in PBMC before and after being stimulated with PHA were detected by biotin-streptavidin (BSA) technique in 188 cases with hepatitis B. Results In the patients with hepatitis B, the levels of CD3~+, CD4~+, and CD4~+/CD8~+were lower, but CD8~+ was higher than those in normal controls (P<0.01): and the total expression levels of mIL-2R in PBMC before and after being stimulated With PHA were also lower than those in normal controls (P<0.01). Of the patients with hepatitis B, the levels of T lymphocyte subsets and mIL-2R in PBMC with HBV-DNA (+) were lower than those with HBV-DNA (-), which were signicantly different (P<0.01). In addition, the levels of mIL- 2R in PBMC were lower in silence than those in inducting with PHA (P<0.01). Conclusions Both cellular and humoral immune functions are obviously disorder in patients with hepatitis B, which might closely associate with the chronicity of the patients with hepatitis B.