| 环氧化酶-2单核苷酸多态性与结直肠癌生物学行为的相关性研究 |
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| 引用本文： | 陈志刚,魏 嘉,禹立霞,钱晓萍,刘宝瑞. 环氧化酶-2单核苷酸多态性与结直肠癌生物学行为的相关性研究[J]. 南京医科大学学报(自然科学版), 2009, 29(7): 963-966 |
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| 作者姓名： | 陈志刚 魏 嘉 禹立霞 钱晓萍 刘宝瑞 |
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| 作者单位： | 南京大学医学院附属鼓楼医院肿瘤中心,南京大学临床肿瘤研究所,江苏,南京,210008 |
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| 摘 要： | 目的:探讨环氧化酶-2(COX-2)基因启动子区-1195G>A单核苷酸多态性与结直肠癌病理参数以及与COX-2表达的关系.方法:对118例结直肠癌血标本,应用SYBR GREEN PCR溶解曲线方法进行COX-2启动子区-1195G>A基因型分析.结果:含有A等位基因的GA或AA基因型相对于GG基因型,淋巴结转移以及肿瘤分期差异显著(p=0.007及P=0.010).GA、AA基因型COX-2阳性率相对于GG基因型OR分别为3.01(95%CI 1.07～8.46,P=0.036)、2.79(95%CI 1.17～6.64,P=0.020).结论:COX-2-1195G>A多态性与淋巴结转移、肿瘤分期以及COX-2表达密切相关,该位点含A等位基因提示淋巴结转移、肿瘤分期晚高风险,COX-2免疫组化阳性率高.
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| 关 键 词： | 环氧化酶-2 单核苷酸多态性 结直肠癌 肿瘤生物学行为 |
| 收稿时间： | 2009-01-19 |
Single nucleotide polymorphism in cyclooxygenase-2 and its association with biological behavior of colorectal cancer |
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| CHEN Zhi-gang,WEI Ji,YU Li-xi,QIAN Xiao-ping and LIU Bao-rui. Single nucleotide polymorphism in cyclooxygenase-2 and its association with biological behavior of colorectal cancer[J]. Acta Universitatis Medicinalis Nanjing, 2009, 29(7): 963-966 |
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| Authors: | CHEN Zhi-gang WEI Ji YU Li-xi QIAN Xiao-ping LIU Bao-rui |
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| Affiliation: | Department of Oncology;Drum Tower Hospital Affiliated to Medical School of Nanjing University & Clinical Cancer Institute of Nanjing University;Nanjing 210008;China |
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| Abstract: | Objective:To examin the single nucleotide polymorphism (SNP) in COX-2 promoter (-1195G>A) and the association between pathological parameters and COX-2 expression in colorectal cancer. Methods:Genotyping of the COX-2 promoter -1195G>A was carried out by single-tube SYBR GREEN PCR with Tm-shift primers. 118 samples of colorectal cancer patients were anaylzed by genotyping. Results:Stratified analysis indicated that an elevated risk of lymph node metastasis and advanced stage was observed in subjects who carr... |
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| Keywords: | PCR |
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