Studies on the effect of sodium arsenate on the enzymes of carbohydrate metabolism,brush border membrane,and oxidative stress in the rat kidney |
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| Affiliation: | 1. Department of Biochemistry, Faculty of Life Sciences, Aligarh Muslim University, Aligarh 202002, UP, India;2. DST-INSPIRE Faculty, Cell Biology & Physiology Division, CSIR-Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, Kolkata 700032, India;3. SVKMs Mithibai College, Bhakti Vedanta Marg, Vile Parle (W), Mumbai 400056, India;1. School of Life Sciences, Shandong University of Technology, Zibo, Shandong Province 255049, China;2. Analysis and Testing Center, Shandong University of Technology, Zibo, Shandong Province 255049, China;1. Department of Biochemistry, School of Medicine, Nantong University, 19 Qi Xiu Rode, 226001 Nantong, China;2. Department of Biosystem Engineering, College of Biosystem Engineering and Food Science, Zhejiang University, 388 Yu Hang Tang Road, 310058 Hangzhou, China;3. Department of Biochemistry and Genetics, School of Medicine, Zhejiang University, 388 Yu Hang Tang Road, 310058 Hangzhou, China;1. School of Public Health, Taishan Medical University, Taian 271000, China;2. School of Public Health, Shandong University, Jinan 250012, China;3. College of Animal Science & Veterinary Medicine, Guangxi University, Nanning 530004, China;4. Key Laboratory of Pathogenic Microbiology and Immunology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China;5. Ruijin Hospital, Shanghai Jiao Tong University, Shanghai 200025, China;1. Institute for Medical Research and Occupational Health, Ksaverska c. 2, 10 001 Zagreb, Croatia;2. University of Zadar, Department of Health Studies, Trg Kneza Višeslava 9, 23 000 Zadar, Croatia;3. Institute of Public Health Zadar, Kolovare 2, 23 000 Zadar, Croatia;4. General Hospital Zadar, Bože Perinčića 5, 23 000 Zadar, Croatia |
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| Abstract: | Arsenic is an environmental pollutant and its contamination in drinking water poses serious world wide environmental health threats. It produces multiple adverse effects in various tissues, including the kidney. However, biochemical mechanism and renal response to its toxic insult are not completely elucidated. We hypothesized that sodium arsenate (ARS) induces oxidative stress and alters the structure and metabolic functions of kidney. Male Wistar rats were administered ARS (10 mg/kg body weight/day), intraperitoneally daily for 10 days. ARS administration increased blood urea nitrogen, serum creatinine, cholesterol, glucose, and phospholipids but decreased inorganic phosphate, indicating kidney toxicity. The activity of brush border membrane (BBM) enzymes significantly lowered in both cortex and medulla. Activity of hexokinase, lactate dehydrogenase, glucose-6-phosphate dehydrogenases, and NADP-malic enzyme significantly increased whereas malate dehydrogenase, glucose-6-phosphatase, and fructose 1,6 bis phosphatase decreased by ARS exposure. The activity of superoxide dismutase, GSH-peroxidase, and catalase were selectively altered in renal tissues along with an increase in lipid peroxidation. The present results indicated that ARS induced oxidative stress caused severe renal damage that resulted in altered levels of carbohydrate metabolism and BBM enzymes. |
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| Keywords: | Sodium arsenate Carbohydrate metabolism Brush border membrane enzymes Oxidative stress Rat kidney |
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