基于Sestrin2通过mTOR信号通路调节胰腺癌上皮-间质转化过程的实验研究

目的 探讨Sestrin 2在胰腺癌上皮-间质转化(EMT)过程中的作用.方法 对胰腺癌细胞PANC-1和CFPAC-1进行Sestrin 2表达干预,通过划痕试验、Transwell试验、RT-PCR、Western blotting和动物实验检测不同Sestrin 2表达水平下胰腺癌EMT过程的变化.同时,分析Se...

Study on the regulation of epithelial-mesenchymal transition in pancreatic cancer by Sestrin 2 through mTOR signaling pathway

Objective To investigate the role of Sestrin 2 in epithelial mesenchymal transition (EMT) of pancreatic cancer PANC-1 and CFPAC-1. Methods The expression of Sestrin 2 in different pancreatic cancer cells was intervened. The changes of EMT were detected by wound healing assay, Transwell invasion assay, RT-PCR, Western blotting and animal test. Meanwhile, the expression of Sestrin 2 in human body was analyzed.Results In the Transwell invasion assay and the wound healing assay, the migration ability and invasion ability of pancreatic cancer cells with high expression of Sestrin 2 were significantly decreased (P<0.05); In the animal test, the tumor growth of pancreatic cancer cells with high expression of Sestrin 2 was significantly decreased (P<0.05); In the RT-PCR and Western blotting analysis, the expression level of E-cadherin was significantly increased (P<0.05), while the expression levels of N-Cadherin and Vimentin were significantly decreased (P<0.05) in the pancreatic cancer cells with high expression of Sestrin 2. The expression of Sestrin 2 was low in human pancreatic cancer; with the increase of Sestrin 2 expression, the expression level of p60-s6k, a key molecule in mTOR signaling pathway, decreased significantly (P<0.05). Conclusion This study suggests that Sestrin 2 can inhibit the EMT of pancreatic cancer, which playing a role through mTOR signaling pathway.