Cytotoxic effects of three new metabolites from Red Sea marine sponge,Petrosia sp. |
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| Institution: | 1. Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, PO Box 80260, Jeddah 21589, Saudi Arabia;2. Department of Marine Chemistry, Faculty of Marine Sciences, King Abdulaziz University, PO. Box 80207, Jeddah 21589, Saudi Arabia;3. Department of Pharmaceutical Chemistry and Phytochemistry, Faculty of Pharmacy, King Abdulaziz University, PO Box 80260, Jeddah 21589, Saudi Arabia;4. Department of Chemistry, Faculty of Science, King Abdulaziz University, PO. Box 80203, Jeddah 21589, Saudi Arabia;6. Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt;7. Department of Chemistry, Faculty of Science, Dammietta University, New Dammietta, Egypt;8. Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt;1. Department of Natural Medicinal Chemistry, China Pharmaceutical University, Nanjing 210009, China;2. Center of Excellence in Post-Harvest Technologies, North Carolina Agricultural and Technical State University, Kannapolis 28081, NC, USA;1. Department of Veterinary Pharmacology, Joint Faculty of Veterinary Medicine, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan;2. Department of Basic Medical Sciences, National Institute for Minamata Disease, 4058-18 Hama, Minamata, Kumamoto 867-0008, Japan;3. Department of Bioscience and Engineering, College of Systems Engineering and Science, Shibaura Institute of Technology, 307 Fukasaku, Minuma-ku, Saitama 337-8570, Japan;4. Laboratory of Pharmacology, School of Pharmaceutical Science, Kitasato University, 5-9-1 Shirokane, Minato-ku, Tokyo 108-8641, Japan;1. Department of Biotechnology, Kurukshetra University, Kurukshetra (Haryana) 136119, India;2. Department of Biochemistry, Kurukshetra University, Kurukshetra (Haryana) 136119, India;3. Department of Microbiology, Kurukshetra University, Kurukshetra (Haryana) 136119, India;1. Anadolu University, Faculty of Sciences, Department of Biology, 26470 Eski?ehir, Turkey;2. Anadolu University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 26470 Eskisehir, Turkey;1. Serik Central Hospital, Medical Biochemistry Department, Antalya, Turkey;2. Suleyman Demirel University, Medical Faculty, Medical Biochemistry Department, Isparta, Turkey;3. Kocaeli University, Medical Faculty, Education of Medicine Department, Kocaeli, Turkey |
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| Abstract: | Marine sponges represent an affluent source of biogenetically unprecedented array of biologically active compounds. This study revealed the isolation of ten compounds from marine sponge of Petrosia sp. Their chemical structures were determined by using 1D and 2D NMR, UV, IR and MS measurements. A polyoxygenated steroid (3β,7β,9α-trihydroxycholest-5-en (1), a purine-derivative (3,7-dimethyl-2-(methylamino)-3H-purin-6(7H)-one (2) and a sphingolipid (N-((3S,E)-1,3-dihydroxytetracos-4-en-2-yl)stearamide (3) proved to be new compounds. Meanwhile, seven known compounds; (4–10) were also identified. The cytotoxicity of the total extract and the isolated compounds were subjected to cytotoxicity evaluation employing two cancer cell lines; HepG2 and MCF-7. All tested compounds exhibited cytotoxic effect on both cancer cell lines with IC50 in range of 20-500 μM. The proposed mechanism of cytotoxic activities was examined through its molecular affinity to the DNA. Compound 5 showed the highest affinity to the DNA with IC50 30 μg/mL. |
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| Keywords: | Sponges Sphingolipids Sterols Cytotoxicity HepG2 MFC-7 |
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