Protective effects of timosaponin B-II on high glucose-induced apoptosis in human umbilical vein endothelial cells期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

按检索

Protective effects of timosaponin B-II on high glucose-induced apoptosis in human umbilical vein endothelial cells

Institution:	1. Department of Sciences, University of Basilicata, Campus di Macchia Romana, Viale dell''Ateneo Lucano 10, 85100 Potenza, Italy;2. Istituto Nazionale di Geofisica e Vulcanologia (INGV), Sezione di Palermo, Via Ugo La Malfa 153, 90146 Palermo, Italy;3. Department of Nature and Land Resources, University of Sassari, Via Piandanna 4, 07100 Sassari, Italy;4. Agenzia Regionale per la Protezione dell''Ambiente di Basilicata, Via della Fisica 18 C/D, 85100 Potenza, Italy;5. National Research Council, Institute of Methodologies for Environmental Analysis, C/da S. Loja, 85050 Tito Scalo (PZ), Italy

Abstract:	This study was designed to investigate the action of timosaponin B-II, a main bioactive compound in Anemarrhena asphodeloides Bunge, on the prevention from high glucose-induced cytotoxicity and apoptosis in human umbilical vein endothelial cells (HUVECs) and the potential mechanisms involved. The results showed that compared with the normal control group, exposure of HUVECs to high glucose media for 72 h resulted in a significant increase in lactates dehydrogenise release, reactive oxygen species production, Caspase-3 activity and the percentage of apoptotic cells (p < 0.01). However, pretreatment with timosaponin B-II significantly increased the viability of HUVECs and decreased lactates dehydrogenise release, Caspase-3 activity and the apoptosis rate in a concentration-dependent manner (p < 0.05). In addition, timosaponin B-II notably decreased the amount of reactive oxygen species and malondialdehyde, as well as promoted glutathione peroxidase activity, endothelial nitric oxide synthase activity and nitric oxide release (p < 0.05). These results suggest that timosaponin B-II has the antiapoptotic effect in endothelial cells through inhibition of high glucose-induced oxidative stress and has the potential for preventing diabetic cardiovascular complications.

Keywords:	Timosaponin B-II Glucose Endothelial cells Reactive oxygen species Caspase-3 Apoptosis BCA"} {"#name":"keyword" "$":{"id":"kw0040"} "$$":[{"#name":"text" "_":"bicinchoninic acid DMEM"} {"#name":"keyword" "$":{"id":"kw0050"} "$$":[{"#name":"text" "_":"Dulbecco's modified Eagle's medium DMSO"} {"#name":"keyword" "$":{"id":"kw0060"} "$$":[{"#name":"text" "_":"dimethylsulfoxide eNOS"} {"#name":"keyword" "$":{"id":"kw0070"} "$$":[{"#name":"text" "_":"endothelial nitric oxide synthase ELISA"} {"#name":"keyword" "$":{"id":"kw0080"} "$$":[{"#name":"text" "_":"enzyme-linked immune-sorbent assay ECGS"} {"#name":"keyword" "$":{"id":"kw0090"} "$$":[{"#name":"text" "_":"endothelial cell growth supplement FBS"} {"#name":"keyword" "$":{"id":"kw0100"} "$$":[{"#name":"text" "_":"fetal bovine serum GSH-Px"} {"#name":"keyword" "$":{"id":"kw0110"} "$$":[{"#name":"text" "_":"glutathione peroxidase HUVECs"} {"#name":"keyword" "$":{"id":"kw0120"} "$$":[{"#name":"text" "_":"human umbilical vein endothelial cells HG"} {"#name":"keyword" "$":{"id":"kw0130"} "$$":[{"#name":"text" "_":"high glucose LDH"} {"#name":"keyword" "$":{"id":"kw0140"} "$$":[{"#name":"text" "_":"lactates dehydrogenise MTT"} {"#name":"keyword" "$":{"id":"kw0150"} "$$":[{"#name":"text" "_":"3-(4 5-dimethylthiazol-2-yl)-2 5-diphenyl tetrazolium bromide MDA"} {"#name":"keyword" "$":{"id":"kw0160"} "$$":[{"#name":"text" "_":"malondialdehyde NO"} {"#name":"keyword" "$":{"id":"kw0170"} "$$":[{"#name":"text" "_":"nitric oxide PBS"} {"#name":"keyword" "$":{"id":"kw0180"} "$$":[{"#name":"text" "_":"phosphate buffered saline ROS"} {"#name":"keyword" "$":{"id":"kw0190"} "$$":[{"#name":"text" "_":"reactive oxygen species
本文献已被 ScienceDirect 等数据库收录！

设为首页 | 免责声明 | 关于勤云 | 加入收藏