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Analysis of Y chromosome haplogroups in Japanese population using short amplicons and its application in forensic analysis

Institution:	1. Biocod Biotecnologia, Av. do Contorno 9636 3th Floor, Santo Agostinho, Belo Horizonte, MG 30110936, Brazil;2. Departamento de Biologia Geral, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Av. Antonio Carlos 6627, Pampulha, Post Office Box 486, Belo Horizonte, MG 31270-910, Brazil;1. Institute of Medical Genetics, the People’s Hospital of Henan Province, #7 Weiwu Road, Jinshui District, Zhengzhou, Henan, China;2. School of Basic Medical Sciences, Zhengzhou University, #100 Kexue Road, Hi-tech Development District, Zhengzhou, Henan, China;1. Department of Forensic Medicine, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan;2. Department of Diagnostic Analysis, Tohoku University Graduate School of Medicine, 2-1 Seiryo-machi, Aoba-ku, Sendai 980-8575, Japan;1. Department of Chemical and Biomolecular Engineering, National University of Singapore, Singapore 117576, Republic of Singapore;2. Department of Civil and Environmental Engineering, National University of Singapore, Singapore 117576, Republic of Singapore

Abstract:	We designed three mini multiplex PCR systems using single-base extension reactions to identify Japanese Y chromosome haplogroups. We selected a group of 22 Y chromosome single nucleotide polymorphisms (SNPs) from the haplogroups most commonly reported in East Asia. To make the systems more useful in analyzing degraded DNA samples, we designed primers to render amplicons of ⩽150 bp. Applying these systems, we classified the Japanese population into major haplogroups and confirmed the applicability of these systems in forensic DNA analysis.

Keywords:	Y chromosome SNPs Multiplex single base extension reactions Degraded DNA Short PCR products
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