Identification of a rare off-ladder allele of the D13S325 locus during paternity testing |
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| Institution: | 1. Instituto de Investigación en Genética Molecular, Universidad de Guadalajara (CUCI-UdeG), Ocotlán, Jalisco, Mexico;2. Laboratorio de Genética Forense, Instituto de Criminalística y Servicios Periciales, Procuraduría General de Justicia, Nuevo León, Mexico;3. Laboratorio de Genética Forense, Servicios Periciales de la Procuraduría General de Justicia del Estado de Baja California, BC, Mexico;4. Laboratorio de Genética del Instituto de Ciencias Forenses, Poder Judicial del Distrito Federal, México DF, Mexico;5. Departamento de Zoología y Antropología Física, Universidad Complutense de Madrid (UCM), 28040 Madrid, Spain;6. Institut Universitari d’Investigació en Ciències de la Salut (IUNICS) i Laboratori de Genètica, Departament de Biologia, Universitat de les Illes Balears, Carretera de Valldemossa, km 7.5, 07122 Palma de Mallorca, Illes Balears, Spain;1. Department of Biochemistry, School of Biological Science and Technology, Central South University, PR China;2. Department of Forensic Medicine, School of Basic Medicine Sciences, Central South University, PR China;3. State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, PR China;4. Department of Internal Medicine, Affiliated Hospital of Inner Mongolia University for Nationalities, PR China;1. GENES SAS Laboratory, Medellín, Colombia;2. Institute of Biology, University of Antioquia, Medellín, Colombia;3. School of Health Sciences, Pontificia Bolivariana University, Medellín, Colombia;4. Laboratorio de Genética Molecular, Cruz Roja Ecuatoriana—Cruz Vital, Quito, Ecuador;5. Centro de Investigación Genética y Genómica, Universidad Tecnológica Equinoccial, Quito, Ecuador;6. Laboratorio de Biología Molecular, Fundación Arthur Stanley Gillow, Bogotá, Colombia;7. IdentiGEN—Genetic Identification Laboratory and Research Group of Genetic Identification, University of Antioquia, Medellin, Colombia;8. Laboratorio de Genética—Universidad Industrial de Santander, Bucaramanga, Colombia;1. Department of Family Genetics, Norwegian Institute of Public Health, P.O. Box 4040, Nydalen, NO-0403 Oslo, Norway;2. Department for Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Aas, Norway |
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| Abstract: | This study demonstrates an unusual rare allele of D13S325 that was falsely categorized as an allele of D12S391 under the STRtyper?-10F/G system. The parentage cases with these rare alleles were analyzed using the Sinofiler? system and singleplex amplification system, and the alleles of D13S325 extracted from the electrophoresis gel were sequenced. 5 Cases with the rare alleles misread as allele 20 of D12S391 were identified in total 2618 cases (including 3200 unrelated parents). This rare allele was designated as allele 5.1 of D13S325 based on its DNA sequence. Its frequency in the Chinese population was 1.6 × 10?3. Because the rare allele 5.1 of D13S325 locus tends to be incorrectly labeled in the STRtyper?-10F/G system, particular attention should be paid when the system is used in paternity testing, personal identification, and DNA database comparisons. |
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| Keywords: | D13S325 Rare allele Paternity testing Personal identification |
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