Frequency distribution of high risk alleles of CYP2C19, CYP2E1, CYP3A4 genes in Haryana population |
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| Affiliation: | 1. Department of Biotechnology, Kurukshetra University, Kurukshetra (Haryana) 136119, India;2. Department of Biochemistry, Kurukshetra University, Kurukshetra (Haryana) 136119, India;3. Department of Microbiology, Kurukshetra University, Kurukshetra (Haryana) 136119, India;1. Department of Natural Products and Alternative Medicine, Faculty of Pharmacy, King Abdulaziz University, PO Box 80260, Jeddah 21589, Saudi Arabia;2. Department of Marine Chemistry, Faculty of Marine Sciences, King Abdulaziz University, PO. Box 80207, Jeddah 21589, Saudi Arabia;3. Department of Pharmaceutical Chemistry and Phytochemistry, Faculty of Pharmacy, King Abdulaziz University, PO Box 80260, Jeddah 21589, Saudi Arabia;4. Department of Chemistry, Faculty of Science, King Abdulaziz University, PO. Box 80203, Jeddah 21589, Saudi Arabia;6. Department of Pharmacognosy, Faculty of Pharmacy, Minia University, Minia 61519, Egypt;7. Department of Chemistry, Faculty of Science, Dammietta University, New Dammietta, Egypt;8. Department of Pharmacognosy, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt;1. Dept of Veterinary Public Health and Food Safety;2. National Center for Immunobiologicals Research and Evaluation, Istituto Superiore di Sanità, Viale Regina Elena, 299, 00161 Rome, Italy;3. The list of members of the RotaNet-Italy Study Group who contributed data is shown in the Appendix;1. Department of Computer Science and Engineering, Yuan Ze University, Taoyuan, Taiwan;2. Innovation Center for Big Data and Digital Convergence, Yuan Ze University, Taoyuan, Taiwan |
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| Abstract: | The genotype of an individual can significantly influence the disposition of a chemical, and determine their susceptibility to its toxicity. Many enzymes involved in either activation or detoxification of chemical carcinogen metabolism are polymorphically expressed, with the alleles presenting different enzymatic activities and some of them having been associated with susceptibility to cancer. Cytochrome P450 (P450 or CYP) constitutes the most important phase I enzyme group responsible for the metabolism of endogenous and exogenous (xenobiotics) substances. The present study was aimed to analyze the frequencies of commonly known polymorphisms of human xenobiotic metabolizing genes (XMG) in the Haryana State population of North India. The study was conducted in 308 healthy Haryana volunteers. DNA was extracted from leucocytes and the genetic polymorphisms in CYP2C19*2, CYP2C19*3, CYP2E1*5B and CYP3A4*1B were determined by digesting the PCR Product with restriction enzymes BamHI, SmaI, PstI and PstI respectively. The genotype frequencies of CYP2C19*2, CYP2C19*3, CYP2E1*5B, and CYP3A4*1B were found to be 22.0%, 0.0%, 2.11% and 2.0% respectively. The North Indian population which is known to be Caucasoid Aryans is ethnically different from South Indians known as Caucasoid Dravidians but no significant difference in genetic polymorphism was found. |
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| Keywords: | Xenobiotic metabolizing genes Genetic polymorphism Haryana population Cytochrome P450 enzymes |
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