Hepatic expression patterns of aryl hydrocarbon receptor,pregnane X receptor,two cytochrome P450s and five phase II metabolism genes responsive to 17alpha-methyltestosterone in rare minnow Gobiocypris rarus |
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| Affiliation: | 1. Post-Graduate Program of Ecology and Natural Resources, Department of Ecology and Evolutionary Biology, Federal University of São Carlos, Rodovia Washington Luis, km 235, CEP 13565-905 São Carlos, SP, Brazil;2. Special Bureau for the Environment, Federal University of São Carlos, Rodovia Washington Luis, km 235, CEP 13565-905 São Carlos, SP, Brazil;3. Department of Physiological Sciences, Federal University of São Carlos, Rodovia Washington Luis, km 235, CEP 13565-905 São Carlos, SP, Brazil;4. Department of Ecology and Evolutionary Biology, Federal University of São Carlos, Rodovia Washington Luis, km 235, CEP 13565-905 São Carlos, SP, Brazil;1. Department of Medicine, James Graham Brown Cancer Center, University of Louisville School of Medicine, Louisville, KY 40202, United States;2. Department of Pharmacology and Toxicology, University of Louisville School of Medicine, Louisville, KY 40202, United States;3. RJ Reynolds Tobacco Co., R&D, P.O. Box 1487, Winston-Salem, NC 27102, United States;1. College of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, China;2. College of Environment, Zhejiang University of Technology, Hangzhou 310032, China |
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| Abstract: | 17Alpha-methyltestosterone (MT), a synthetic androgen, is widely used in aquaculture. Aquatic organisms can receive continuous exposure to residual MT throughout their lives. Aiming to evaluate the effects of MT on genes involved in biotransformation pathway, meanwhile attempting to unravel the MT metabolic pathway at the transcriptional level in fish, here we isolated the cDNAs of previously unreported AHR2, Sult1 st1, Ugt2a1 and Ugt2b6 in rare minnow, and predominantly investigated the hepatic transcriptional patterns of AHR2, PXR and five biotransformation genes after MT exposure in both genders adult rare minnow Gobiocypris rarus. The present findings suggest that AHR2 and PXR should play important roles in regulating biotransformation enzymes related to MT catabolism, moreover, CYP1A, CYP3A, SULT1 ST4, SULT1 ST6 and UGT2A1 may play certain roles in catabolism of MT in adult G. rarus. Additionally, UGT2A1 may make greater contribution than SULT1 ST4 and SULT1 ST6 in MT catabolism in males. |
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| Keywords: | Cytochrome P450 Sulfotransferase UDP-glucuronosyltransferases 17Alpha-methyltestosterone |
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