Variability in DNA damage of chub (Squalius cephalus L.) blood,gill and liver cells during the annual cycle |
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| Affiliation: | 1. Department of Natural Resources and Environmental Sciences, Institute for Multidisciplinary Research, Kneza Višeslava 1, University of Belgrade, Belgrade, Serbia;2. Center for Genotoxicology and Ecogenotoxicology, Chair of Microbiology, Faculty of Biology, Studenski Trg 16, University of Belgrade, Belgrade, Serbia;3. Institute for Biological Research “Siniša Stanković”, Despota Stefana 142, University of Belgrade, Belgrade, Serbia;1. Department of Functional Diagnostic Science, Osaka University Graduate School of Medicine, Osaka, Japan;2. Department of Nephrology, Nagoya University Graduate School of Medicine, Nagoya, Japan;3. Departments of Geriatric Medicine and Nephrology, Osaka University Graduate School of Medicine, Osaka, Japan;4. Department of Specific Organ Regulation, Osaka University Graduate School of Medicine, Osaka, Japan;5. Department of Advanced Technology for Transplantation, Osaka University Graduate School of Medicine, Osaka, Japan;1. Department of Materials Science and Engineering, National Chung Hsing University, Taichung 40227, Taiwan;2. Material and Chemical Research Laboratories, Industrial Technology Research Institute, Chutung 30010, Taiwan;1. Graduated School of Engineering, Yokohama National University, Tokiwadai 79-5, Hodogaya, Yokohama 240-8501, Japan;2. Faculty of Engineering, Yokohama National University, Tokiwadai 79-5, Hodogaya, Yokohama 240-8501, Japan;1. Institute of Chemistry, Karl-Franzens-University Graz, Stremayrgasse 16, 8010 Graz, Austria;2. Department of Chemistry, Addis Ababa University, Addis Ababa, Queen Elizabeth II St., Ethiopia |
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| Abstract: | In this work the genotoxic potential of water in three localities in Serbia, which differ by the nature and degree of pollution, was determined in tissues of European chub (Squalius cephalus L.) on monthly basis over the 2011/2012 year season using the alkaline comet assay. Specimen samples of chub were taken from Special Nature Reserve “Uvac”, as control site, and Pestan and Beljanica Rivers, as polluted sites at Kolubara basin, surrounded with coal mines. Three tissues, blood, gills and liver were used for assessing the level of DNA damage. Analysis was done by software (Comet Assay IV). The control site at Reserve “Uvac” showed the lowest DNA damage values for all three tissues compared to Pestan and Beljanica. Blood has the lowest level of DNA damage in comparison with liver and gills. Decreased damage for all three tissues was observed at summer, while during the spring and autumn damage increased. |
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| Keywords: | Genotoxicity Comet assay European chub Water pollution |
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