| 越橘花色苷对宫颈癌Hela细胞凋亡及抗氧化能力的影响 |
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| 引用本文： | 姜艳霞,芦晓静,徐俊杰,吕士杰.越橘花色苷对宫颈癌Hela细胞凋亡及抗氧化能力的影响[J].上海中医药杂志,2011(9):62-65. |
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| 作者姓名： | 姜艳霞 芦晓静 徐俊杰 吕士杰 |
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| 作者单位： | 吉林医药学院生物化学教研室; |
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| 基金项目： | 吉林省教育厅课题(2006126) |
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| 摘 要： | 目的探讨越橘花色苷对宫颈癌Hela细胞的作用机制。方法以不同浓度的越橘花色苷作用于宫颈癌Hela细胞48h后，通过Gimsa染色观察越橘花色苷抑制Hela细胞的形态学改变，通过流式双染观察Hela细胞凋亡情况，通过RT．PCR观察Hela内P53的表达，并测定Hela细胞内的SOD、GSH、MDA值。结果Gimsa染色可见越橘花色苷可使Hela细胞光泽度下降，细胞数减少，稀疏，细胞变小、核固缩。流式双染可见，随着橘花色苷浓度升高，凋亡率升高。与对照组比较，越橘花色苷浓度达到30mg／ml时，P53的表达量最高，差异有统计学意义（P〈0．05）。与对照组比较，30mg／ml和40mg／ml的越橘花色苷组SOD和GSH含量升高，差异有统计学意义（P〈0．05）。结论越橘花色苷可通过上调抑癌基因P53的表达，增强细胞凋亡，并提高Hela细胞的抗氧化能力，从而达到抑制宫颈癌Hela细胞的作用。
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| 关 键 词： | 越橘花色苷 细胞凋亡 P53 SOD GSH MDA |
Effects of Anthocyanin from Vaccinium uliginosum on cell apoptosis and antioxidation in cervical Hela cells |
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| JIANG Yan-xia,LU Xiao-jing,XU Jun-jie,LU Shi-jie Section of Biochemistry,Jilin Pharmacological College.Effects of Anthocyanin from Vaccinium uliginosum on cell apoptosis and antioxidation in cervical Hela cells[J].Shanghai Journal of Traditional Chinese Medicine,2011(9):62-65. |
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| Authors: | JIANG Yan-xia LU Xiao-jing XU Jun-jie LU Shi-jie Section of Biochemistry Jilin Pharmacological College |
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| Institution: | JIANG Yan-xia,LU Xiao-jing,XU Jun-jie,LU Shi-jie Section of Biochemistry,Jilin Pharmacological College |
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| Abstract: | Objective To research the mechanisms of Anthocyanin from Vaccinium uliginosum on proliferation of cervical cancer Hela cells.Methods Different concentrations of Anthocyanin from Vaccinium uliginosum on Hela cells was done for 48 hours to observe its inhibiting cervical cancer Hela cells and the change of cell morpholoy by Gimsa dying was used to observe cell apoptosis by flow cytometry(Annexin V FITC/PI)and to detect P53 expression by RT-PCR,and detect SOD,GSH and MDA in Hela cells.Results Increasing concen... |
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| Keywords: | Anthocyanin cell apoptosis P53 SOD GSH MDA |
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