高效液相色谱法测定咪喹莫特乳膏的含量 |
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引用本文: | 曹琳,周征,毛亚珠.高效液相色谱法测定咪喹莫特乳膏的含量[J].中国药品标准,2008,9(3):226-227. |
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作者姓名: | 曹琳 周征 毛亚珠 |
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作者单位: | 浙江省宁波市药品检验所,宁波315040 |
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摘 要: | 目的:建立HPLC法测定咪喹莫特乳膏的含量。方法:采用Zorbax C18(4.6mm×150mm,5μm)色谱柱,流动相为乙腈-0.02mol·L^-1磷酸氢二钾缓冲液(用磷酸调节pH至8.0)(30:70),流速为1.0mL·L^-1,检测波长为245nm。结果:咪喹莫特的线性范围为0.05—26.10mL·L^-1(r=0.9999)。平均加样回收率(n=9)为99.4%,RSD=0.2%。结论:该方法简便、可靠、准确,可用于该制剂的质量控制。
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关 键 词: | 咪喹莫特乳膏 HPLC 液相色谱法 含量 |
Determination of Imiquimod Cream by HPLC |
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Authors: | Cao Lin Zhou Zheng and Mao Ya-zhu |
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Institution: | (Ningbo Institute for Drug Control , Ningbo 315040) |
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Abstract: | Objective:To establish a method of HPLC for determination of Imiquimod Cream. Methods:A Zothax C18 (4. 6 mm × 150 mm,5 μm ) column was used. Acetonitrile -0. 02 mol · L^-1 potassium phosphate dibasic was used as the mobile phase, the flow rate was 1.0 mL ·min^-1. The detection wavelength was 245 nm. Results:The linear range of Imiquimod was 0. 05 - 26. 10 mg· L^-1 ( r =0. 999 9 ). The average recovery was 99. 4%, with RSD =0. 2% (n =9). Conclusion:The method is simple, reliable, accurate and can be applied to the quality control of the preparation. |
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Keywords: | HPLC |
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