|
|||||
|
|
Role of substance P in several models of bladder inflammation |
|
| Authors: | Judith Luber-Narod Tammy Austin-Ritchie Carl Hollins III Mani Menon Rajwant K Malhotra Stephen Baker Robert E Carraway |
| Institution: | 1. Department of Surgery, University of Massachusetts Medical School, 55 Lake Avenue North, 01655, Worcester, MA, USA 2. Cambridge Bioscience, Worcester, Massachusetts, USA 3. BASF, Inc., Worcester, Massachusetts, USA 4. Department of Pathology, University of Massachusetts Medical Center, Worcester, Massachusetts, USA 5. Department of Information and Research, University of Massachusetts Medical Center, Worcester, Massachusetts, USA 6. Department of Cellular and Molecular Physiology, University of Massachusetts Medical Center, Worcester, Massachusetts, USA |
| Abstract: | Substance P (SP) is a peptide found in the sensory nervous system which has multiple biologic effects including stimulation of muscle contraction, pain nociception, immune cell functions, plasma extravasation and a constellation of inflammatory effects. Here we investigate the role of SP in several animals models of bladder inflammation. Using the female Lewis rat, inflammation was induced using either xylene, lipopolysaccharide (LPS) or polyinosinic-polycytidylic acid (polyIC). Inflammation occurred rapidly (4 h) and was maintained in each model for at least 7 days. Each of these protocols decreased the bladder content of immunoreactive SP by approximately 50%, suggesting enhanced release. There was no change in the urinary frequency of these animals over 3 weeks, suggesting that urinary frequency changes are not mediated by acute inflammation. We also found that the SP receptor (NK1) antagonist, (?)CP96345, was unable to block the inflammation produced by polyIC, suggesting that SP is not an obligatory mediator of immune cell stimulation in this model. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |