表情肌肌纤维组织化学分裂及分类研究

目的 对活体表情肌组织化学特点进行研究，为面部表情肌动脉复活提供实验依据。方法 标本均取自头面颈部手术病人切口下和的表情肌，并在拟冷切片机中切片后进行组织化学染色，包括肌动球蛋白ＡＴＰ酶（ＭＡＴＰａｓｅ）及 的型畏酶１－四唑氮蓝（ＮＡＤＡ－ＴＲ），结果依靠计算机辅助图像分析。结果 各表情肌肌纤维直径在２４．３～６３．９μｍ之间不等，表情肌中同一型肌纤维直径不相同，Ｐ〈０．０５，各表情肌中各型肌数量

Histochemical study and classification of facial muscles fibers]

OBJECTIVE: To study the histological and histochemical characteristics of the facial muscle fiber. METHODS: Seven biopsy specimens of the facial muscle from twelve patients were stained with histochemical method. The diameter and the histochemical type of the fibers were analysed by staining of M-ATP ase and NADA-TR. Type I and type II fibers were evaluated respectively by means of computer-assisted image analysis. RESULTS: The fiber diameter is between 24.3-63.9 microns. According to the quantity of fiber I, facial muscles may be classified into three groups: 1. Phasic muscle contains less than 20 percent of type I fibers; 2. Intermediate muscle contains 21 to 40 percent of type I fibers; 3. Tonic muscle contains 41 to 69 percent of type I fibers. CONCLUSION: The results of the study show that the histological and histochemical difference of facial muscles may have significance in facial paralysis restoration.