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| 热休克蛋白70在百草枯诱导的肺部炎性反应中的表达 | |
| 引用本文： | 李景栋,金抗,杨云鹤,于杉,孙俊萍,金阗.热休克蛋白70在百草枯诱导的肺部炎性反应中的表达[J].中国医科大学学报,2010,39(3). |
| 作者姓名： | 李景栋 金抗 杨云鹤 于杉 孙俊萍 金阗 |
| 作者单位： | 1. 中国医科大学,附属第一医院急诊科,沈阳,110001 2. 北京市顺义区医院,急诊科,北京,101300 3. 中国医科大学,附属第一医院神经内科,沈阳,110001 4. Texas Heart Institute,St,Luke's Episcopal Hospital,Houston,Tx 77030,USA 5. 日本冈山大学齿科,冈山700-8525,日本 |
| 摘 要： | 目的 探讨百草枯中毒致急性肺损伤中热休克蛋白70(HSP70)的表达.方法 72只健康Wistar大鼠随机平均分成3组,分别腹腔注射生理盐水(A组)、20 mg/kg百草枯(B组)和40 mg/kg百草枯(C组).分别于1,2,3,6,12和24 h留取标本,应用免疫组织化学染色、Western blot方法测定肺组织中HSP70的蛋白表达.结果 B组和C组大鼠肺组织发生急性肺损伤.B组大鼠2 h后HSP70表达与A组相比开始增强,6 h为表达高峰,12 h表达减少,24 h表达水平与A组相当.C组1 h后HSP70表达与A组相比明显增强,3 h达到高峰,24 h后与A组水平一致.结论 百草枯诱导的急性肺损伤可引起支气管、细支气管和肺泡上皮细胞HSP70应激性表达,提示HSP70对肺损伤起保护作用. |
| 关 键 词： | 百草枯中毒 热休克蛋白70 肺损伤 |
Expression of Heat Shock Protein-70 in Paraquat-induced Pulmonary Inflammation Response |
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| LI Jing-dong , JIN Kang , YANG Yun-he , YU Shan , SUN Jun-ping , JIN Tian.Expression of Heat Shock Protein-70 in Paraquat-induced Pulmonary Inflammation Response[J].Journal of China Medical University,2010,39(3). | |
| Authors: | LI Jing-dong JIN Kang YANG Yun-he YU Shan SUN Jun-ping JIN Tian |
| Abstract: | Objective To investigate the expression of heat shock protein-70(HSP70)in acute lung injury induced by paraquat poisoning. Methods A total of 72 Wistar rats were randomly divided into 3 groups (n =24 each group)and intraperitoneally injected with normal saline in group A,20 mg/kg paraquat in group B,and 40 mg/kg paraquat in group C. The lungs of rats were excised 1,3,6,12,and 24 hours after the injection,respectively. The expression of HSP70 in the lung tissue was detected by immunohistochemistry and Wester... |
| Keywords: | paraquat poisoning heat shock protein-70 lung injury |
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