大鼠肝脏再生过程中细胞标志物演变与骨髓细胞增生

Changes of bone marrow cells and cell markers in regenerated liver after partial hepatectomy in rats

AIM: To investigate the replication and mobilization of bone marrow (BM) cells in support of liver regeneration, and the alteration of hepatic oval cell markers in the regenerated liver. METHODS: In the Sprague-Dawley (SD) rats receiving 2/3 partial hepatectomy (PHx) with or without 2-acetylamin-ofluorene (2AAF) treatment, CD34+, CD45+ BM cells were measured by flow cytometry. The changes in cell markers of hemopoietic stem cells, hepatic oval cells and hepato-cytes in the regenerated liver were determined by fluorescence immunohistochemistry at the indicated time points after PHx. RESULTS: The numbers of CD34+ and CD45+ BM cells were increased in both PHx (2-6 d) and PHx+2AAF (2-4 d) rats. CD34 and CD45 co-expressing cells were observed in the regenerated liver sections, which was significantly increased after 2AAF treatment. In the PHx+2AAF rats, the cell markers co-expressed with CD34 exhibited dynamic changes: Thy1.1 appeared first, followed by AFP, vimentin, CK19, and Alb; while Thy1.1, Alb, and CK19 disappeared earlier than AFP and vimentin. CONCLUSION: CD34+, CD45+ BM cells replicate during the liver regeneration in rats. The changes of the cell markers co-expressed with CD34 reflect the in vivo development of hepatic oval cells.