STZ诱导的2型与1型糖尿病大鼠模型的对比研究

目的 观察链脲佐菌素（STZ）诱导的2型与1型糖尿病大鼠模型的差异。方法 雄性SD大鼠，高脂高糖饲养6周后按35mg/kg单次腹腔注射2%STZ溶液建立2型糖尿病（T2DM）模型，普通饲料饲养6周后按60mg/kg单次腹腔注射2%STZ溶液建立1型糖尿病（T1DM）模型，设立正常对照组。检测大鼠体重、血糖、糖化血红蛋白（HbA1c）、血脂谱及氧化型低密度脂蛋白（ox-LDL）水平。结果 与正常组比较，糖尿病组大鼠的血糖、血脂和ox-LDL水平显著升高（P<0.01）；T1DM组体重显著下降（P<0.01），T2DM组体重差异无统计学意义（P>0.05）。T2DM组的体重、血脂谱及ox-LDL水平显著高于T1DM组（P <0.01），血糖差异无统计学意义（P >0.05）。结论 高脂、高糖喂养基础上单次腹腔注射STZ可成功诱导2型糖尿病大鼠模型，其体重及血脂异常显著高于1型糖尿病模型，近似于人类2型糖尿病。

Comparison of Type 2 and Type 1 Diabetic Rats Induced by Streptozotocin

Objective To observe the difference between type 2 and type 1 diabetic rats induced by streptozotocin (STZ).Methods Male SD rats, after 6 weeks'' high fat and high sugar diet and then with a single intraperitoneal injection of 2%STZ solution by dose of 35mg/kg to esbabish type 2 diabetes mellitus (T2DM) model, common diet for 6 weeks to esbabish type 1 type 1 diabetes mellitus (T1DM) model by single intraperitoneal injection of 60mg/kg 2%STZ.And the normal control group is also esbabished. The body weight, blood sugar, glycated hemoglobin (HbA1c), blood lipid spectrum and oxidized low density lipoprotein (ox-LDL) level were measured.Results Compared with the normal group, the blood glucose, lipid and ox-LDL levels in the diabetic group increased significantly (P<0.01), the weight in the T1DM group decreased significantly (P<0.01), and there was no significant difference in the T2DM group (P>0.05). The body weight, blood lipid spectrum and ox-LDL level in T2DM group were significantly higher than those in T1DM group (P<0.01). There was no significant difference in blood sugar between T1DM and T2DM groups (P>0.05).Conclusion A single intraperitoneal injection of STZ in rats on the basis of high fat and high glucose diet can induce type 2 diabetes model similar to human type 2 diabetes, and their body weight and blood lipid abnormalities are significantly higher than those of type 1 diabetes.