红花提取物对肝纤维化大鼠肝线粒体的作用

目的 探讨红花提取物对肝纤维化大鼠肝细胞线粒体的保护作用。方法 二乙基亚硝胺诱导大鼠肝纤维化模型，实验组口服红花提取物，分离各组肝组织细胞线粒体，观察肝细胞线粒体形态学改变，检测MDA和SOD在肝细胞线粒体中的水平。观察线粒体膜势能和线粒体ATP的水平改变，并观察线粒体的呼吸功能变化，以研究红花提取物对肝纤维化大鼠肝细胞线粒体是否具有保护作用。结果 正常组大鼠肝线粒体排列整齐、形态正常，对照组的大鼠线粒体肿胀且形态不规则，边界不清晰，且大小不一致，而实验组线粒体的肿胀、结构不清晰、大小不一等情况有明显改善；肝纤维化大鼠肝细胞线粒体中MDA的水平明显升高，而SOD的含量明显下降，红花提取物处理则能明显降低线粒体MDA的水平，并提高SOD的含量；与正常组相比，肝纤维化大鼠的线粒体膜势能明显降低，而红花提取物处理能够提高线粒体膜势能；大鼠由于肝纤维化可加重消耗肝细胞线粒体中的ATP，而红花提取物能明显减少大鼠线粒体中的ATP消耗。与之一致的是，实验组大鼠线粒体中的磷氧比明显高于对照组。结论 红花提取物能明显降低大鼠肝纤维化诱导的肝细胞线粒体氧化应激。能维持肝细胞线粒体ATP水平、呼吸控制率及磷氧比来保护线粒体的功能。

Effects of Safflower Extract on Liver Tissue Cell Mitochondria of Hepatic Fibrosis in Rats

Objective To investigate the protective effect of safflower extract on hepatocyte mitochondria in hepatic fibrosis rats.Methods Rat model of hepatic fibrosis was induced by diethyl nitrosamine. Oral safflower extract was taken orally in the experimental group. Hepatic mitochondria were isolated from each group. Morphological changes of hepatocyte mitochondria were observed and MDA and SOD were detected in the level hepatocyte mitochondria. Observe changes in mitochondrial membrane potential and mitochondrial ATP levels and observe changes in mitochondrial respiratory function to investigate whether safflower extract has protective effects on hepatocyte mitochondria in rats with hepatic fibrosis or not.Results In the normal group, the rat mitochondria were arranged neatly and the morphology was normal. In the control group, the mitochondria of the rats were swollen with irregular morphology, the borders were unclear, and the sizes were inconsistent. In the experimental group, the mitochondria were swollen, the structures were not clear, and the sizes were different. The above condition is significantly improved; liver fibrosis in rats'' mitochondria MDA levels increased significantly, while SOD content decreased significantly, safflower extract treatment can significantly reduce mitochondrial MDA levels, and it can increase SOD content. Compared with the normal group, the mitochondrial membrane potential of liver fibrosis rats was significantly reduced, while the safflower extract treatment can increase the mitochondrial membrane potential; due to liver fibrosis, rats can increase the consumption of ATP in liver mitochondria and safflower extract can significantly reduce ATP consumption in rat mitochondria. Consistent with this, the phosphor-to-oxygen ratio in the rat mitochondria of the experimental group was significantly higher than that of the control group.Conclusion The safflower extract can significantly reduce the hepatocyte mitochondrial oxidative stress induced by hepatic fibrosis in rats. It can maintain mitochondrial ATP level, respiratory control rate and phosphorus-oxygen ratio to protect mitochondrial function.