| miR-29家族在深低温停循环相关性神经元损伤中的作用 |
| |
| 引用本文： | 李红梅,葛俊文,张儒舫,沈立. miR-29家族在深低温停循环相关性神经元损伤中的作用[J]. 医学研究杂志, 2018, 47(5): 28-31,35 |
| |
| 作者姓名： | 李红梅 葛俊文 张儒舫 沈立 |
| |
| 作者单位： | 200062 上海交通大学附属儿童医院、上海市儿童医院心胸外科,200062 上海交通大学附属儿童医院、上海市儿童医院心胸外科,200062 上海交通大学附属儿童医院、上海市儿童医院心胸外科,200062 上海交通大学附属儿童医院、上海市儿童医院心胸外科 |
| |
| 基金项目： | 国家自然科学基金资助项目（81371449） |
| |
| 摘 要： | 目的 观察miR-29家族在低温糖氧剥夺/再灌注（oxygen-glucose deprivation/reoxygenation，OGD/R） HT22细胞中的表达，进而探究miR-29家族在深低温停循环（deep hypothermic circulatory arrest，DHCA）相关性神经元死亡中的特异性作用及其相关机制。方法 将HT22细胞随机分为对照组、低温OGD/R组、类似物组（agomir-NC组、agomir-29a组、agomir-29b组和agomir-29c组）和抑制剂组（antamir-NC组、antamir-29a组、antamir-29b组和antamir-29c组）；运用一个密闭容器和一个厌氧产气袋建立OGD/R模型；定量PCR检测HT22细胞内miR-29家族的表达；CCK-8方法检测活细胞数目；Western blot法检测HT22细胞内Bax和PUMA蛋白含量；JC-1和H2DCFDA法分别测定HT22细胞内活性氧（reactive oxygen species，ROS）含量和线粒体膜电位（mitochondrial membrane potential，MMP）。结果 低温OGD/R模型中miR-29家族表达显著下降（P<0.01）。miR-29家族过表达显著抑制了低温OGD/R诱导的HT22细胞死亡，以及Bax和PUMA蛋白的表达（P均<0.01）；同时减轻了ROS含量和MMP水平（P均<0.01）。结论 miR-29家族可以通过减轻氧化应激从而对DHCA介导的神经元损伤产生保护性作用。
|
| 关 键 词： | 深低温停循环 糖氧剥夺/再灌注 miR-29 氧化应激 |
| 收稿时间： | 2017-09-04 |
| 修稿时间： | 2017-09-06 |
Role of miR-29 Family in the DHCA-associated Neuron Injury |
| |
| Li Hongmei,Ge Junwen,Zhang Rufang. Role of miR-29 Family in the DHCA-associated Neuron Injury[J]. Journal of Medical Research, 2018, 47(5): 28-31,35 |
| |
| Authors: | Li Hongmei Ge Junwen Zhang Rufang |
| |
| Affiliation: | Department of Cardiothoracic Surgery, Shanghai Children''s Hospital, Shanghai Jiao Tong University, Shanghai 200062, China,Department of Cardiothoracic Surgery, Shanghai Children''s Hospital, Shanghai Jiao Tong University, Shanghai 200062, China,Department of Cardiothoracic Surgery, Shanghai Children''s Hospital, Shanghai Jiao Tong University, Shanghai 200062, China and Department of Cardiothoracic Surgery, Shanghai Children''s Hospital, Shanghai Jiao Tong University, Shanghai 200062, China |
| |
| Abstract: | Objective To investigate the expression and role of miR-29 family in HT22 cells with low temperature OGD/R, and further explore the specific role and its related mechanism of miR-29 family in DHCA-related neuron death.Methods The HT22 cells were randomly divided into the control group, low temperature OGD/R group, analogue group (agomir-NC group, agomir-29a group, agomir-29b group and agomir-29c group), inhibitor group(antamir-NC group, antamir-29a group, antamir-29b group and antamir-29c group). The OGD/R model was established using an airtight container and an anaeropack. The expression of miR-29 family on HT22 cell was determined by quantitative PCR. CCK-8 kit was used to detect the number of living cells. Western blot was conducted for the quantification of Bax and PUMA proteins. JC-1 and H2DCFDA were used to measure reactive oxygen species (ROS) level and mitochondrial membrane potential (MMP) of HT22 cells, respectively.Results The expression of miR-29 family was significantly declined in low temperature OGD/R model (P<0.01). Overexpression of miR-29 family significantly alleviated the HT22 cell injury induced by low temperature OGD/R, and inhibited the expression of Bax and PUMA protein (P<0.05). In addition, the injection of miR-29 family analogues inhibited the increase of ROS and MMP in low temperature OGD/R model (P<0.05).Conclusion MiR-29 family could exert its protective effect against OGD/R-mediated HT22 cell injury by reducing oxidative stress. |
| |
| Keywords: | Deep hypothermic circulatory arrest Oxygen-glucose deprivation/reoxygenation miR-29 Ooxidative stress |
|
| 点击此处可从《医学研究杂志》浏览原始摘要信息 |
|
点击此处可从《医学研究杂志》下载全文 |
|
