支架蛋白JLP基因敲除小鼠的构建及其肾脏表型的初步观察

目的 繁殖和鉴定支架蛋白JLP基因敲除小鼠，对其肾脏表型进行初步观察。方法 将JLP基因敲除杂合小鼠进行配种繁殖，提取子代小鼠的组织DNA，用PCR技术检测小鼠的基因型，并用Western blot法验证基因敲除效果。通过PAS病理染色等观察肾脏微观结构。结果 成功繁殖并获得子代小鼠，子代小鼠有3种基因型，包括纯合子（JLP+/+、JLP-/-）和杂合子（JLP+/-）。PCR检测基因型的方法方便且结果准确。结论 笔者成功建立了JLP基因敲除小鼠模型，能够进一步探究发现JLP基因对生物体的作用及机制。

Construction of a Mouse Model for Scaffold Protein JLP Knockout and Preliminary Observation of the Renal Phenotype

Objective To breed the scaffolding protein JLP knockout mice and observe the renal phenotype. Methods Breeding the JLP heterozygous mice will get the homozygous mice offspring. PCR reaction was used to detect the gene genotype after extracting the mice tissue DNA and the microstructure of the kidney was observed by PAS pathological staining. Results The homozygous mice offspring were obtained and 3 genotypes including JLP+/+, JLP+/-and JLP-/- were tested. Conclusion JLP knockout mice provides an effective animal model for further research on the function of JLP protein.