CCR10的表达对非小细胞肺癌细胞在增殖、迁移和侵袭的影响

目的 本研究旨在探讨趋化因子受体10（chemokine receptor 10，CCR10）的表达对NSCLC细胞在增殖、迁移和侵袭方面的影响。方法 应用基因工程技术将CCR10siRNA导入NSCLC细胞中，干扰CCR10的正常表达，进而研究CCR10对NSCLC细胞在增殖、迁移和侵袭等功能方面的影响。结果 转染了CCR10siRNA的人NSCLC腺癌细胞A549的细胞增殖率明显低于空白对照组和阴性对照组，差异有统计学意义（P<0.05），空白对照组与阴性对照组的细胞增殖率比较，差异无统计学意义（P>0.05）。与空白对照组及阴性对照组比较，转染了CCR10siRNA的人NSCLC腺癌细胞A549的细胞迁移率明显低于空白对照组及阴性对照组，差异有统计学意义（P<0.05），空白对照组和阴性对照组的细胞迁移率比较，差异无统计学意义（P>0.05）。转染了CCR10siRNA的人NSCLC腺癌细胞A549的细胞侵袭率明显低于空白对照组及阴性对照组，差异有统计学意义（P<0.05），空白对照组及阴性对照组的细胞侵袭率比较，差异无统计学意义（P>0.05）。结论 CCR10促进NSCLC细胞的增殖、迁移和侵袭能力，CCR10可能参与非小细胞肺癌的发生、发展、侵袭及转移过程。

Effects of CCR10 Expression on Proliferation, Migration and Invasion of NSCLC Cells

Objective To investigate the effects of CCR10 expression on proliferation, migration and invasion of NSCLC cells.Methods CCR10 siRNA was conducted into humanlung adenocarcinoma cellA549(A549) to interfere with the normal expression ofCCR10, and then to study how dose CCR10 have effects on cell proliferation, migrationand invasion in NSCLC.Results The results of MTT showed that the proliferation rate of A549 conducted withCCR10 siRNA was significantly decreased compared with Negative Control(NC) and Mock(P<0.05). The proliferation rate of A549 in NC compared with Mock has nostatistical significance (P>0.05).The results of Transwell cell migration assay showed that the migration rate ofA549 conducted with CCR10 siRNA was significantly decreased compared with NC and Mock(P<0.05). The proliferation rate of A549 in NC compared with Mock has nostatistical significance (P>0.05).The results of Transwell cell invasion assay showed that the invasion rate ofA549 conducted with CCR10 siRNA was significantly decreased compared with NC and Mock(P<0.05). The invasion rate of A549 in NC compared with Mock has nostatistical significance (P>0.05).Conclusion CCR10 may contribute to cell proliferation, migration and invasion in NSCLC,and associated with the occurrence and development of NSCLC.