泛素化特异性蛋白酶39在结肠癌组织中的表达及临床意义

目的 检测泛素化特异性蛋白酶39（ubiqutin-specific protease，USP39）在结肠癌组织中的表达情况，并分析USP39的表达水平与77例结肠癌患者的临床病理特征、生存及预后之间的相关性。方法 利用免疫组织化学技术检测结肠癌石蜡组织标本中USP39蛋白的表达情况，利用卡方分析结肠癌组织及癌旁组织中USP39的表达差异，利用Spearman秩相关分析USP39与结肠癌患者临床病理特征的相关性，利用K-M生存曲线分析USP39与结肠癌患者5年总体生存率的相关性，利用Cox风险比例模型分析USP39与结肠癌患者预后的相关性。结果 免疫组织化学结果显示USP39在结肠癌组织中的阳性率显著高于癌旁组织；Spearman秩相关分析显示USP39的表达水平与结肠癌患者TNM分期及淋巴转移情况呈正相关，而与其他临床病理特征无显著相关性；K-M生存曲线结果显示USP39阳性表达的结肠癌患者术后5年总体生存率低于USP39阴性表达的结肠癌患者（P=0.009）。Cox风险比例模型结果显示USP39可作为独立判断结肠癌患者预后的分子指标。结论 USP39在结肠癌组织中表达升高，可作为预测结肠癌患者生存周期及预后的潜在分子标志物。

Expression and Clinical Significance of USP39 in Colon Cancer

Objective To explore the expression of USP39 in tissues of colon cancer (CRC), investigate the association of the expression levels of USP39 and clinicopathological features of CRC patients, and analyze the relevance of USP39 and survival and prognosis of CRC patients. Methods Expressions of USP39 were analyzed by immunohistochemistry in 77 CRC patients. Spearman''s rank test, K-M survival curves, and Cox proportional hazards risk were conducted to analyze the clinical relevance of USP39 in CRC. Results Immunohistochemistry revealed that the positive rate of USP39 was upregulated in CRC tissues compare with adjacent tissues Spearman rank correlation showed that positive USP39 expression was significantly associated with TNM stage, lymph node status and venous invasion of CRC patients. Kaplan-Meier curves showed that positive USP39 expression was inversely correlated with 5 years overall and progression-free survival time of CRC patients. Cox proportional hazards risk analysis revealed that USP39 was an independent prognostic factor for CRC. Conclusion USP39 expression is upregulated in tissues of CRC, and USP39 is a potential survival and prognosis biomarker in CRC.