N-cadherin、E-cadherin和P63蛋白在原发性肝细胞癌组织中的表达及临床意义

目的 探讨神经型钙黏蛋白（N-cadherin）、上皮型钙黏蛋白（E-cadherin）以及P63蛋白在原发性肝细胞癌（HCC）组织中的表达及其与患者临床病理学特征之间的相关性。方法 采用免疫组化染色法分别检测60例正常肝组织、60例HCC组织及其癌旁组织标本中N-cadherin、E-cadherin以及P63蛋白的表达，并分析HCC组织中三者表达与患者临床病理学特征的关系。结果 N-cadherin在正常肝组织、癌旁组织、肝癌组织中的阳性表达率逐渐升高，差异均有统计学意义（P<0.05）。而E-cadherin在正常肝组织、癌旁组织、肝癌组织的阳性表达率则逐渐降低，差异均有统计学意义（P<0.05），正常组织的P63阳性表达率显著高于癌旁组织和HCC组织，差异有统计学意义（P<0.05），而癌旁组织与HCC组织间比较差异无统计学意义（P>0.05）。N-cadherin的高表达与HCC患者的高病理学分级、门静脉受侵犯、高临床分期等结局有显著相关性，差异有统计学意义（P<0.05）。E-cadherin的低表达与HCC患者的高病理学分级、门静脉受侵犯、高临床分期等结局有显著相关性，差异有统计学意义（P<0.05）。P63的低表达与HCC患者的高病理学分级、肿瘤直径、高临床分期等结局有显著相关性，差异有统计学意义（P<0.05）。P63的表达与E-cadherin的表达呈正相关（r=0.467，P<0.01），而与N-cadherin的表达呈负相关（r=-0.472，P<0.01）。结论 HCC组织中N-cadherin的高表达和P63蛋白、E-cadherin的低表达可能与肿瘤的发生、发展及转移密切相关。

Expressions of N-cadherin, E-cadherin and P63 Protein in Hepatocellular Carcinoma and Their Clinical Significance

Objective To explore the expressions of N-cadherin, E-cadherin, and P63 protein in hepatocellular carcinoma (HCC) tissues, and the relationship between their expressions and clinicopathologic factors.Methods Immuohistochemistry was used to detect the expressions of N-cadherin, E-cadherin, and P63 protein in 60 normal liver tissues, 60 HCC tissues and their peficancerous tissues, and then the relationship between their expressions and clinicopathologic factors were analyzed.Results The positive expression rates of N-cadherin in normal liver tissue,peficancerous tissues and HCC tissue were gradually decreased, the difference was statistically significant (P<0.05). And The positive expression rates of E-cadherin in normal liver tissue,peficanceroustissues and HCC tissue were gradually increased, the difference was statistically significant (P<0.05).The expression of P63 in normal liver tissue was significantly higher than that in peficancerous tissues and HCC tissue, and the differences were all significant(P<0.05).The positive rates of expression of N-cadherin were related with clinical stage, portal vein invasion,metastasis and pathological grading. The positive rates of expression of E-cadherin were related with clinical stage, portal vein invasion and pathological grading. The positive rates of expression of P63 were related with clinical stage, tumor sizeand pathological grading. Spearman rank correlation analysis showed that the expression of P63was positively correlated with the expression of E-cadherin, but negatively correlated with the expression of N-cadherin.Conclusion The high expression of N-cadherin and low expression of P63 protein, E-cadherin of HCC tissues may play a role in tumor metastasis and carcinogenesis.