The murine submandibular gland conditioned medium induce the development of CFU-GM and CFU-meg]

OBJECTIVE: Our purpose is to determine whether murine submandibular gland produce hematopoietic growth factors or not and which hematopoietic growth factor are produced by murine submandibular gland. METHODS: By using the techniques of culture of hematopoietic progenitor cells(CFU-GM, CFU-Meg) ex vivo, flow-cytometry, the effects of murine submandibular gland conditioned medium(SGCM) on the development of CFU-GM and CFU-Meg in mice was studied. RESULTS: SGCM can stimulate the development of CFU-GM and CFU-Meg (without any exogeneous HGF), almost no colony in control (without any exogeneous HGF and SGCM); the promoting activity of male SGCM is higher than female SGCM's in augmenting colony formation of CFU-Meg (P < 0.05), there is no difference of CFU-GM(P > 0.05). The stimulating activity of anemic mice SGCM is higher normal mice SGCM's (P < 0.05). IL-3 can collaborate with female SGCM to stimulate proliferation of CFU-Meg. SGCM can promote bone morrow cell entiring the active proliferative phase(S/G2). CONCLUSION: Submandibular gland can stimulate the development of CFU-GM and CFU-Meg by secreting hematopoietic growth factor-like activities. Our study provide the experimental evidences for clarifying the relationship between submandibular gland and hematopoiesis.