| 同源盒基因IRX1的启动子克隆及其在人胃黏膜细胞中的启动活性分析 |
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| 引用本文： | 郭晓波,郭磊,吴建林,刘卫仁,计骏,张佳年,刘炳亚,朱正纲,于颖彦.同源盒基因IRX1的启动子克隆及其在人胃黏膜细胞中的启动活性分析[J].中华胃肠外科杂志,2010,13(11):846-850. |
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| 作者姓名： | 郭晓波 郭磊 吴建林 刘卫仁 计骏 张佳年 刘炳亚 朱正纲 于颖彦 |
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| 作者单位： | 上海消化外科研究所,上海交通大学医学院附属瑞金医院外科,200025 |
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| 基金项目： | 国家自然科学基金，国家863重大项目，上海市科委重点基础研究项目，上海市浦江人才计划项目，上海市慈善基金会癌症研究专项基金 |
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| 摘 要： | 目的 克隆同源盒基因IRX1的核心启动子序列,并探讨其转录调控机制.方法 通过生物信息学预测IRX1基因启动子范围：利用PCR方法扩增并构建含有IRX1基因启动子不同片段的真核报告基因质粒,瞬时转染GES-1胃黏膜细胞,通过检测不同片段的荧光素酶报告质粒活性,判断不同片段的启动子活性.结果 生物信息学预测IRX1基因启动子位于转录起始点上游700 bp范围内.通过PCR技术扩增获得IRX1基因5＇侧翼区8个截短片段报告基因质粒,其活性由大到小依次为p-416＞p-584＞p-715＞p-350＞p-687＞p-320＞p-188＞p-92 除p-92与p-188片段外,其余各片段与PGL3-basic空载体的差异均有统计学意义（均P＜0.05）,其中以p-416片段活性最强,片段p-320与片段p-188之间出现较大活性落差.结论 IRX1基因上游序列启动子以p-416转录活性最高,核心启动子位于-320～-188区间,该区间具有Sp1、TFⅡD等转录因子结合序列,是下一步转录调控机制研究的重要位点.
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| 关 键 词： | 基因 IRX1 启动子 报告基因 胃黏膜细胞 |
Promoter cloning and activity analysis for iroquois homeobox gene 1 in gastric mucosa cell line |
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| GUO Xiao-bo,GUO Lei,WU Jian-lin,LIU Wei-ren,JI Jun,ZHANG Jia-nian,LIU Bing-ya,ZHU Zheng-gang,YU Ying-yan.Promoter cloning and activity analysis for iroquois homeobox gene 1 in gastric mucosa cell line[J].Chinese Journal of Gastrointestinal Surgery,2010,13(11):846-850. |
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| Authors: | GUO Xiao-bo GUO Lei WU Jian-lin LIU Wei-ren JI Jun ZHANG Jia-nian LIU Bing-ya ZHU Zheng-gang YU Ying-yan |
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| Institution: | GUO Xiao-bo(Department of Surgery,Ruijin Hospital,Shanghai Jiaotong University School of Medicine, Shanghai Institute of Digestive Surgery,Shanghai 200025,China) GUO Lei(Department of Surgery,Ruijin Hospital,Shanghai Jiaotong University School of Medicine, Shanghai Institute of Digestive Surgery,Shanghai 200025,China) WU Jian-lin(Department of Surgery,Ruijin Hospital,Shanghai Jiaotong University School of Medicine, Shanghai Institute of Digestive Surgery,Shanghai 200025,China) LIU Wei-ren(Department of Surgery,Ruijin Hospital,Shanghai Jiaotong University School of Medicine, Shanghai Institute of Digestive Surgery,Shanghai 200025,China) JI Jun(Department of Surgery,Ruijin Hospital,Shanghai Jiaotong University School of Medicine, Shanghai Institute of Digestive Surgery,Shanghai 200025,China) ZHANG Jia-nian(Department of Surgery,Ruijin Hospital,Shanghai Jiaotong University School of Medicine, Shanghai Institute of Digestive Surgery,Shanghai 200025,China) LIU Bing-ya(Department of Surgery,Ruijin Hospital,Shanghai Jiaotong University School of Medicine, Shanghai Institute of Digestive Surgery,Shanghai 200025,China) ZHU Zheng-gang(Department of Surgery,Ruijin Hospital,Shanghai Jiaotong University School of Medicine, Shanghai Institute of Digestive Surgery,Shanghai 200025,China) YU Ying-yan(Department of Surgery,Ruijin Hospital,Shanghai Jiaotong University School of Medicine, Shanghai Institute of Digestive Surgery,Shanghai 200025,China) |
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| Abstract: | |
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| Keywords: | Iroquois homeobox gene 1 Promoters Reporter genes Gastric mucosa cell line |
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