食管癌及癌前病变患者血清中多个自身抗体检测的研究

目的:探讨多个肿瘤相关抗原微阵列在食管癌及癌前病变患者血清自身抗体检测中能否提高癌和癌前病变患者的检出率。方法:肿瘤相关抗原微阵列包含8个重组的癌抗原蛋白C-myc,p53,cyclinB1,p16,p62,Koc,IMP1,Survivin,它们分别来自相应的cDNA文库的表达。应用间接酶联免疫反应法检测863例食管不同病理类型血清中的自身抗体。结果:在8个抗原中,p53、C-myc、IMP1、p16、Koc和Survivin在食管各级病变中阳性百分率具有线性趋势。在食管各级病变中,尤其是Survivin,从正常到癌其阳性百分率分别是:10%、10%、20%、100%,正常和DYS和SCC之间差异显著。单一抗体的反应频率均较低,当应用多个抗原分析时,即在8个肿瘤相关抗原中至少有一个反应为阳性时,本研究的阳性率明显增高,即:10%、30%、50%和100%,正常与各级病变之间差异显著(P<0.05)。结论:联合应用多个肿瘤相关抗原比应用单个肿瘤相关抗原分析食管癌及癌前病变患者血清中的自身抗体变化更能够提高癌和癌前病变患者的检出率。食管癌特异性的多个相关抗原微阵列的进一步优化有可能作为临床上食管肿瘤和高危人群检测和诊断的非侵袭性方法。

Multiple autoantibody detection in esophageal precancerous or cancerous serum using tumor-associated antigens mini-array

Objective:To determine whether it might enhance the detection frequency of patients with esophageal or precancerous lesion by using a mini-array of tumor-associated antigens (TAAs) to detect the autoantibody of serum.Methods:The TAA mini-array consists of eight full-length recombinant proteins expressed form respective cDNA library encoding C-myc,p53,cyclinB1,p62,p16,Koc,IMP1,Survivin.Enzyme-linked immunoassay was used to profile the autoantibody response relative to eight tumor-associated antigens in 863 sera from subjects ranging from normal to different grades of lesions.Results:All of 8 antigens,the detection frequency of p53、C-myc、IMP1、p16、Koc and Survivin have linear correlation from normal subject upto each steps of disease during the multi-step oncogenesis of esophagus;especially for Survivin which presents 10%、10%、20% and 100% from normal to cancer during the multi-step oncogenesis of esophagus.There are significant differences from normal to more serious pathological changes,including DYS and SCC.Detection of auto-antibody frequency to any individual TAA was very lower.Otherwise,there was a stepwise increase of percentages with the successive addition of individual index which was 10%、30%、50% and 100% in esophagus.There were significant differences from normal to every grade of disease (P<0.05).Conclusion:It might better to enhance the detection rate of esophageal and precancerous lesion through testing multi-TAAs to analying auto-antibodies in serum of patient than one antigen.The future design of multi-antigens mini-arrays unique for esophageal might contribute a clinically potential noninvasive approach to cancer detection and diagnosis.