不同时程吗啡依赖戒断大鼠海马CA1区BDNF 及PSD-95的表达

目的观察不同时程吗啡依赖大鼠戒断1周后海马CA1区脑源性神经生长因子(BDNF)及突触后致密质95(PSD-95)的表达变化,探讨吗啡依赖戒断对海马CA1区的影响。方法建立不同时程(1周、2周、4周)吗啡依赖大鼠模型并自然戒断1周后,应用RT-PCR方法观察海马CA1区BDNF及PSD-95的表达情况。结果吗啡依赖1周戒断组大鼠海马CA1区的BDNF及PSD-95表达较生理盐水对照组下降(P<0.01),吗啡依赖2周戒断组大鼠海马CA1区的BDNF及PSD-95表达较吗啡依赖1周戒断组大鼠有所上升(P<0.05),但仍低于生理盐水对照组(P<0.01),吗啡依赖4周戒断组大鼠海马CA1区的BDNF及PSD-95表达较吗啡依赖1周戒断组大鼠、吗啡依赖2周戒断组大鼠均下降(P<0.01)。结论BDNF及PSD-95在吗啡依赖大鼠自然戒断1周后的海马CA1区的表达降低,吗啡依赖戒断对海马CA1区损伤明显。

The Expression of BDNF and PSD-95 in Hippocampal CA1 Region of Morphine-withdrawn Rat with Different Dependent Times

Objective To observe the expression of brainderived neurotrophic factor(BDNF) and postsynaptic density-95(PSD-95) in hippocampal CA1 region of rat with morphine dependence for different times and withdrawn for 1 week,and investigate the influence of that morphine dependence is withdrawn on rat hippocampal CA1 area.Methods Animal models of rats with morphine withdrawal for 1 week and different morphine dependent times(1 week,2 weeks,4 weeks) were established.The expression of BDNF and PSD-95 in hippocampal CA1 were identified with RT-PCR.Results The expression of BDNF and PSD-95 in hippocampal CA1 decreased in the withdrawn group with morphine dependence for 1 week as compared with that in normal saline(NS) group(P<0.01),and it increased in the withdrawn group with morphine dependence for 2 weeks as compared with that in morphine-dependent group for 1 week(P<0.05) but still decreased as compared with that in NS group(P<0.01), and it decreased in the withdrawn group with morphine dependence for 4 weeks as compared with the other three groups(P<0.01).Conclusion The expression of BDNF and PSD-95 in hippocampal CA1 decreases in morphine-depended rats withdrawn for 1 week.Morphine withdrawal has a manifest harm in hippocampal CA1 region of rat.