兔脂肪肝内VX2移植瘤动物模型建立研究

目的探讨脂肪肝与正常肝内VX2移植瘤模型的建立方法,旨在为临床脂肪肝肿瘤的研究提供依据。方法选用健康家兔34只,随机分为脂肪肝组(n=18)和正常对照组(n=16)。脂肪肝组采用高脂饲料+酒精喂养,正常对照组采用基础饲料+自来水喂养,建立脂肪肝与正常肝内VX2移植瘤模型,应用常规超声及超声造影观察并与病理结果对照。结果⑴高脂饲料+酒精喂养法可成功建立兔脂肪肝模型,成模率100%。⑵开腹肝内组织块种植法可成功建立兔脂肪肝及正常肝内VX2移植瘤模型,成瘤率89.7%。⑶超声造影测量VX2移植瘤最大径与大体病理测值比较,无统计学差异(P>0.05)。结论使用高脂饲料+酒精喂养可成功建立近似人类病理生理过程的脂肪肝模型。开腹组织块种植法建立兔VX2移植瘤模型方法简单,成瘤率高。

The study of establishing fatty liver VX2 tumor model in rabbit

Objective To explore establishing method of VX2 tumors in fatty liver and normal liver, Aimed at providing the basis for clinical fatty liver cancer research. Methods 34 rabbits were randomly divided into fatty liver group（n=18） and control group（n=16）, Animals in fatty group were feeding high-fat diet and drinking water containing 5% ethanol, Animals in control group were feed regular diet and water. The model of fatty liver and normal liver VX2 tumor in rabbit were established, furthermore, compared with pathologic histology. Results （1）Models of fatty liver disease could be established in rabbits by feeding them high-fat and alcohol drink, which the success rate was 100%. （2）The successful rate of implantation VX2 tumor in fatty liver and normal liver of rabbit was 89.7%. （3）Measurements of tumor using contrast-enhanced ultrasound were consistent with pathology, showed no significant difference（P〉0.05）. Conclusion The animal model of fatty liver established by being fed a high-fat and alcohol diet was similar as human pathophysiological process fatty liver. The method implanting VX2 tumor lump to rabbit fatty liver was easy reproduction and high success rate.