探讨游离脂肪酸对近曲肾小管上皮细胞HK-2的影响及作用机制

目的探讨游离脂肪酸（Free Fatty Acids,FFAs）对人近曲肾小管上皮细胞（HK-2）的影响及作用机制。方法用含有不同浓度（0、125、250、500μmol/L）软脂酸的DMEM-F12培养人近曲肾小管上皮细胞（HK-2）,在0h、24h、48h三个时段：MTT法检测不同环境下FFAs对HK-2细胞增殖的影响,流式细胞术检测FFAs对细胞凋亡和细胞周期的影响,免疫细胞化学法检测处理前后HK-2细胞中白细胞介素-β（IL-β）、白细胞介素-8（IL-8）表达的变化。结果 FFAs呈剂量和时间依赖性抑制HK-2细胞的增殖（P〈0.05）,并可能通过阻滞细胞周期,诱导细胞凋亡（P〈0.05）。经FFAs作用后,IL-β、IL-8的表达增加。结论游离脂肪酸有抑制HK-2细胞增殖,促进其凋亡的作用,同时炎性细胞因子IL-β、IL-8表达增多,参与了其发生和发展的过程。

Explore free fatty acids to proximal convoluted renal tubular epithelial cells of HK-2 influence and function mechanism

Objective Explore free fatty acid（FFAs） to proximal convoluted tubules epithelial cells（HK-2） influence and function mechanism.Methods People proximal convoluted tubules epithelial cells（HK-2）,were cultured with DMEM-F12 of different concentrations of palmitic acid（0,125,250.500μmol）（using defat bovine serum albumin as vector）,normal medium as control grop.on the 0 hour,24 hour,48 hour respectively： Applyied four methyl azo thiazole blue（determined by MTT） colorimetric detection different environment HK-2 cell proliferation capacity;Streaming cells in cell apoptosis FFAs intraoperative detection and the influence of the cell cycle;Immune cell chemical method before and after treatment were detected in HK-2 cells interleukin-β（IL-β）,interleukin-8（IL-8） expression changes.Results In FFAs doses and time dependence inhibit HK-2 cell proliferation（P 0.05）,and possibly by blocking the cell cycle and apoptosis（P 0.05）.After FFAs role by IL-β,IL-8 expression increase.Conclusion Free fatty acid HK-2 it inhibits cell proliferation,promote its apoptosis role,and inflammatory cells factor IL-β,IL-8 express increasing,participated in its occurrence and development process.