探讨游离脂肪酸对近曲肾小管上皮细胞HK-2的影响及作用机制 |
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引用本文: | 韩乐,刘晓玲,荣青峰,刘琳. 探讨游离脂肪酸对近曲肾小管上皮细胞HK-2的影响及作用机制[J]. 中国医疗前沿, 2013, 0(5): 23-26 |
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作者姓名: | 韩乐 刘晓玲 荣青峰 刘琳 |
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作者单位: | 山西医科大学第二临床医学院内分泌内科,太原030001 |
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基金项目: | 山西省留学归国人员科研基金(编号:200372) |
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摘 要: | 目的探讨游离脂肪酸(Free Fatty Acids,FFAs)对人近曲肾小管上皮细胞(HK-2)的影响及作用机制。方法用含有不同浓度(0、125、250、500μmol/L)软脂酸的DMEM-F12培养人近曲肾小管上皮细胞(HK-2),在0h、24h、48h三个时段:MTT法检测不同环境下FFAs对HK-2细胞增殖的影响,流式细胞术检测FFAs对细胞凋亡和细胞周期的影响,免疫细胞化学法检测处理前后HK-2细胞中白细胞介素-β(IL-β)、白细胞介素-8(IL-8)表达的变化。结果 FFAs呈剂量和时间依赖性抑制HK-2细胞的增殖(P〈0.05),并可能通过阻滞细胞周期,诱导细胞凋亡(P〈0.05)。经FFAs作用后,IL-β、IL-8的表达增加。结论游离脂肪酸有抑制HK-2细胞增殖,促进其凋亡的作用,同时炎性细胞因子IL-β、IL-8表达增多,参与了其发生和发展的过程。
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关 键 词: | 游离脂肪酸 肾小管上皮细胞 白细胞介素-1β 白细胞介素-8 凋亡 增殖 |
Explore free fatty acids to proximal convoluted renal tubular epithelial cells of HK-2 influence and function mechanism |
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Affiliation: | HAN Le, LIU Xiao-lin, RONG Qing-feng, et al. (The Second Affiliated Medical University, Taiyuan 030001, China ) |
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Abstract: | Objective Explore free fatty acid(FFAs) to proximal convoluted tubules epithelial cells(HK-2) influence and function mechanism.Methods People proximal convoluted tubules epithelial cells(HK-2),were cultured with DMEM-F12 of different concentrations of palmitic acid(0,125,250.500μmol)(using defat bovine serum albumin as vector),normal medium as control grop.on the 0 hour,24 hour,48 hour respectively: Applyied four methyl azo thiazole blue(determined by MTT) colorimetric detection different environment HK-2 cell proliferation capacity;Streaming cells in cell apoptosis FFAs intraoperative detection and the influence of the cell cycle;Immune cell chemical method before and after treatment were detected in HK-2 cells interleukin-β(IL-β),interleukin-8(IL-8) expression changes.Results In FFAs doses and time dependence inhibit HK-2 cell proliferation(P 0.05),and possibly by blocking the cell cycle and apoptosis(P 0.05).After FFAs role by IL-β,IL-8 expression increase.Conclusion Free fatty acid HK-2 it inhibits cell proliferation,promote its apoptosis role,and inflammatory cells factor IL-β,IL-8 express increasing,participated in its occurrence and development process. |
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Keywords: | Free fatty acids Renal tubular epithelial cells Interleukin-1β Interleukin-8 Apoptosis Proliferation |
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