人胃癌组织块裸鼠原位移植/转移模型的建立

 目的　用肿瘤组织块原位移植 ,建立人胃癌裸小鼠原位移植 /转移模型。方法　以人胃低分化腺癌细胞系接种于裸小鼠皮下 ,形成稳定传代的皮下移植瘤 ,再取该肿瘤组织块原位移植于裸鼠胃壁 ,观察移植肿瘤的生长状况、移植成功率和自发转移的发生率。结果　原位移植成功率 (成瘤率 )为 1 0 0 %、局部淋巴结转移率 1 0 0 %、远处淋巴结转移率 90 %、肝转移发生率为 75%。荷瘤鼠的中位生存期为 1 4周 ,晚期出现消瘦和全身衰竭。结论　该裸小鼠原位移植 /转移模型的生物学行为与人胃癌自然生长和转移过程相似 ,可作为一种有价值的工具用于胃癌转移机理和抗转移实验治疗的研究。

Establishment ofOrthotopic Implant/Metastatic Model of Human Stomach Cancer using Intact Tumor Tissue inNude Mice

Objective To develop nude mouse orthotopic implant/metastatic model of human gastric cancer using intact tumor tissue.Methods Tumor cell line of human gastric adenocarcinoma was inoculated subcutaneously into nude mice to develop implant|tumor.Intact tumor tissue was then collected and implanted orthotopically into the nude mouse stomach.The local tumor growth characteristics,tumor|take rates and metastasis rates were examined.Results A 100% tumor|take rate was obtained in the model.The metastases of local and distant lymph nodes were observed in 100% and 90% of the nude mice respectivcely.The liver metastases were found in 75% of the nimals.The median survival time of the tumor|bearing nude mice was 14 weeks.Emacication and exhaustion of the nude mice were presented in late stage of the experiment.ConclusionThe growth and metastasis characteristics of human stomach cancer were exhibited in the orthotopic implant/metastatic model.This tumor model might be a useful tool in the researches on metastatic mechanisms and antimetastasis treatment of human stomach cancer.