| VITEK-2 AST-GN13药敏卡检测产超广谱β-内酰胺酶菌的可靠性研究 |
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| 引用本文： | 郭良,张思萍.VITEK-2 AST-GN13药敏卡检测产超广谱β-内酰胺酶菌的可靠性研究[J].检验医学与临床,2011,8(2):174-175. |
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| 作者姓名： | 郭良 张思萍 |
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| 作者单位： | 福建省宁德市闽东医院检验科,355000 |
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| 摘 要： | 目的评价VITEK-2 AST-GN13药敏卡检测产超广谱β-内酰胺酶(ESBLs)菌株的可靠性。方法用VITEK-2 AST-GN13测定376株肠杆菌科菌(包括大肠埃希菌、肺炎克雷伯菌、产酸克雷伯菌),同时用美国临床实验室标准化委员会(2009年第19版)推荐的双纸片确证法进行对照。结果检出产ESBLs菌160株,仪器法和双纸片确证法检测ESBLs菌株阳性率分别为42.6%和39.4%,两种方法对产ESBLs株的检测结果经χ2检验,差异无统计学意义(P>0.05)。结论提高产ESBLs菌株的阳性检出率及准确性具有重要意义,VITEK-2 AST-GN13药敏卡检测产ESBLs菌株结果可靠。
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| 关 键 词： | β内酰胺酶类 大肠杆菌 克雷伯菌 微生物敏感性试验 |
Study on reliability of VITEK-2 AST-GN13 on detection of ESBLs-producing strains |
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| GUO Liang,ZHANG Si-ping.Study on reliability of VITEK-2 AST-GN13 on detection of ESBLs-producing strains[J].Laboratory Medicine and Clinic,2011,8(2):174-175. |
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| Authors: | GUO Liang ZHANG Si-ping |
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| Institution: | GUO Liang,ZHANG Si-ping(Department of Clinical Laboratory,Mindong Hospital of Ningde City,Ningde,Fujian 355000,China) |
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| Abstract: | Objective To evaluate the reliability of VITEK-2 AST-GN13 on detection of ESBLs-producing strains.Methods 376 strains of Enterobacteriaceae bacteria(including Escherichia coli,Klebsiella pneumoniae,Klebsiella oxytoca) were detected by VITEK-2.At the same time,the double disks confirmation method recommended by NCCLS(19 edition,2009) was used as control.Results 160 strains of ESBLs-producing bacteria were detected.The positive detection rates of ESBLs producing bacteria in the two methods were 42.6% and 39.4... |
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| Keywords: | beta-lactamases Escherichia coli Klebsiella microbial sensitivity tests |
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