温郁金对荷肿瘤裸鼠抑瘤作用和MMP-2、CD44v6表达影响的观察

目的观察温郁金对胃癌细胞抑制作用和MMP-2、CD44v6蛋白表达的影响,探讨其抗胃癌细胞转移的作用机制。方法以SGC-7901胃癌细胞株建立胃癌裸鼠原位移植瘤模型,将裸鼠随机分为对照组(0.9%氯化钠溶液)及实验组(温郁金水煎剂)。观察裸小鼠胃癌种植后肿瘤生长及转移灶情况,用免疫组化法检测2组肿瘤组织中MMP-2和CD44v6蛋白的表达。结果 2组荷瘤鼠胃壁均有肿瘤生长,荷瘤率100%,对照组瘤重(4.09±1.17)g,实验组瘤重(2.73±0.92)g(与对照组比较P<0.05),抑瘤率为33.25%;实验组肝、腹腔和淋巴结转移较生理盐水对照组明显减少(P<0.05);实验组瘤体中MMP-2和CD44v6的表达明显下调。结论温郁金具有抑制人胃癌裸鼠原位移植瘤生长和转移的作用,其作用机制可能与下调MMP-2和CD44v6的表达有关。

Observation the inhibitory effect and expression of MMP-2,CD44v6 of common turmeric among tumor-bearing nude mice

Objective To study the inhibitory effect of common turmeric and the expression of MMP-2 and CD44v6 proteins in human gastric SGC-7901cell,explore the possible mechanisms on gastric cancer metastasis.Method Established nude mouse orthotopic transplantation mode of SGC-7901 and then randomly divided the nude mouse into control group and common turmeric group.The tumor growth and metastasis were observed,the expression of MMP-2 and CD44v6 proteins in the tumor tissue were detected by immunohistochemistry.Results The rate of successfully orthotopic transplantation was 100%.The weight of the tumors in common turmeric group was(2.73±0.92)g,in control group was(4.09±1.17)g,there was statistical significance between the two group(P<0.05),The inhibitary rate of common turmeric group was 33.25%.The metastasis of cavitas peritonealis,liver and lymph node in common turmeric group were significantly lower than those of control group(P<0.05).Meanwhile,we found that the positive rates of MMP-2 and CD44v6 expression in the common turmeric group were obviously lower than that in control group(P<0.05).Conclusions Common turmeric can inhibit gastric cancer growth and metastasis in orthotopic transplantation model of nude mice,the mechanism might be related to down-regulation of MMP-2 and CD44v6 expression.