miR-155在肝细胞癌中的表达及对肝癌细胞增殖的影响

目的:检测微小RNA-155(miR-155)在肝癌组织中的表达并分析其对肝癌细胞增殖和细胞凋亡的影响.方法:采用TagMan MGB探针法荧光定量P C R分析42例原发性肝癌及对应的癌旁组织miR-155的表达;利用miR-155反义寡核苷酸(ASO-miR-155)降低肝癌细胞HepG2和SMMC7721中miR-155的表达;利用MTT比色法检测肝癌细胞增殖的变化,并通过流式细胞技术检测肝癌细胞早期凋亡情况.结果:42例肝癌及癌旁组织标本中,miR-155在52%(22/42)肝癌组织中的表达明显高于癌旁组织(P<0.05);利用脂质体将ASO-miR-155转染肝癌细胞HepG2和SMMC7721后,miR-155的表达明显降低,肝癌细胞HepG2和SMMC7721生长受到明显抑制;并且细胞的早期凋亡明显增加.结论:miR-155在肝癌组织中过表达,降低其表达能明显抑制肝癌细胞的生长并诱导细胞早期凋亡,miR-155有可能成为肝癌治疗的新靶点.

Expression of miR-155 in primary hepatocellular carcinoma and its effect on tumor cell proliferation

AIM:To detect the expression of microRNA 155(miR-155) in hepatocellular carcinoma and to analyze its influence on tumor cell proliferation and apoptosis.METHODS:The expression of miR-155 in 42 cases of primary liver cancer and matched tumor-adjacent normal tissue was detected by quantitative PCR.The expression of miR-155 in hepatocellular carcinoma cell lines HepG2 and SMMC7721 was inhibited using a miR-155-specific antisense oligonucleotide(ASO-miR-155),and cell proliferation and early apoptosis were then determined by MTT assay and flow cytometry,respectively.RESULTS:The positive rate of miR-155 expression was significantly higher in hepatocellular carcinoma(52%) than in tumor-adjacent tissue(P < 0.05).After ASO-miR-155 was transfected into HepG2 and SMMC7721 cells using Lipofectamine,the expression of miR-155 was significantly reduced.MiR155 knockdown significantly inhibited growth and promoted early apoptosis of HepG2 and SMMC7721 cells.CONCLUSION:MiR-155 is overexpressed in hepatocellular carcinoma.Inhibition of miR-155 expression could inhibit growth and induce early apoptosis of hepatocellular carcinoma cells.MiR-155 may become a new target for the treatment of liver cancer.