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| 线粒体在蓝舌病毒HbC3株诱导人肝癌细胞凋亡中的作用 | |
| 引用本文： | 汪艳,易有荣,崔冶建,董长垣.线粒体在蓝舌病毒HbC3株诱导人肝癌细胞凋亡中的作用[J].武汉大学学报(医学版),2006,27(4):425-428,F0002. |
| 作者姓名： | 汪艳 易有荣 崔冶建 董长垣 |
| 作者单位： | 1. 武汉大学医学院病毒学国家重点实验室,武汉,430071;武汉大学医学结构生物学研究中心,武汉,430071 2. 武汉大学医学结构生物学研究中心,武汉,430071 3. 武汉大学医学院病毒学国家重点实验室,武汉,430071 |
| 摘 要： | 目的:探讨BTV-HbC3对人肝癌Hep-3B细胞线粒体功能和膜电位的影响及其与细胞凋亡关系。方法:利用透射电镜和荧光显微镜观察Hep-3B细胞感染BTV-HbC3在24,36,48 h的凋亡情况与其线粒体形态变化;噻唑蓝(MTT)法检测其线粒体功能;流式细胞仪检测经Rh123/PI染色的BTV-HbC3诱导该细胞线粒体跨膜电位(△Ψm)和Annexin V/PI染色的细胞凋亡率。结果:Hep-3B细胞感染病毒在24,36,48 h时可导致线粒体功能下降,同时使线粒体膜电位显著降低,细胞凋亡率显著增加。结论:BTV-HbC3通过诱导凋亡来抑制人肝癌细胞的生长,其机制与线粒体跨膜电位下降有关。 |
| 关 键 词： | 蓝舌病毒HbC3 细胞凋亡 线粒体 膜电位 人肝癌细胞 |
| 文章编号： | 1671-8852（2006）04-0425-04 |
| 收稿时间： | 2005-12-12 |
| 修稿时间： | 2005-12-12 |
Role of Mitochondria in Human Hepatic Carcinoma Cell Apoptosis Induced by Bluetongue Virus HbC3 Strain |
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| WANG Yan,YI Yourong,CUI Yejian,DONG Changyuan.Role of Mitochondria in Human Hepatic Carcinoma Cell Apoptosis Induced by Bluetongue Virus HbC3 Strain[J].Medical Journal of Wuhan University,2006,27(4):425-428,F0002. | |
| Authors: | WANG Yan YI Yourong CUI Yejian DONG Changyuan |
| Institution: | 1,State Key Laboratory of Virology ; 2, Research Center of Medicine and Structural Biology, School of Medicine, Wuhan University, Wuhan 430071 , China |
| Abstract: | Objective: To explore the changes of in human hepatic carcinoma cells Hep-3B after bluetongue virus HbC_3(BTV-HbC_3) infection and their relationship with the BTV-HbC_3 induced apoptosis.Methods: Morphological changes of apoptosis and mitochondria of Hep-3B cells infected with BTV-HbC_(3)were observed under fluorescence microscope and transmission electron microscope.Mitochondrial function was assessed with MTT.The mitochondrial transmembrane potential of Hep-3B cells straining Rh123/PI and the apoptosis of Hep-3B cell staining Annexin V/PI were measured by flow cytometry(FCM).Results: Mitochondrial transmembrane potential and mitochondrial function of Hep-3B cells were significantly decreased at 24,36 and 48 hours after infection,but the rate of Hep-3B cells apoptosis markedly increased.Conclusion: BTV-HbC_(3)might inhibit the Hep-3B cells growth by inducing apoptosis and its mechanism might be related to the loss of △Ψm. |
| Keywords: | Bluetongue Virus HbC3 Apoptosis Mitochondria Transmembrane Potential Human Hepatic Carcinoma Cell |
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