SWH液对大鼠肝脏能量代谢的影响

目的通过与UW液进行比较,探讨SWH液对SD大鼠肝脏能量代谢的影响.方法应用大鼠原位肝移植模型,通过高效液相色谱法(HPLC),检测肝组织ATP、ADP、AMP,计算总腺苷酸含量(TAN)及Atkinson′s能量转换(EC);肝脏低温保存8、12、18 h后行原位肝移植,观察移植术后7 d动物存活率.结果保存18 h组的ATP、TAN、EC恢复水平明显低于保存8 h组,相差显著,保存12 h组与保存8 h组比较相差不显著;SWH液组移植术后7 d动物存活率有高于UW液组的趋势.肝脏ATP、TAN含量及EC水平与大鼠术后生存率的变化趋势相同.结论肝脏ATP、TAN含量及EC水平与大鼠术后生存率密切相关,采用SWH液保存肝脏,能够有效的保护肝脏能量代谢功能.

Effects of SWH solution on hepatic energy metabolism in rats

Objective To investigate the effects of SWH solution on hepatic energy metabolism in SD rats compared with UW solution. Methods Levels of ATP, ADP, and AMP in hepatic tissues were examined by high performance liquid chromatography (HPLC) while ALT, AST, and LDH were examined and the 7-day survival rate of animals was observed with orthotopic liver transplantation model in rats. Results Levels of ATP, TAN, and EC in 18-h group were significant lower than those in 8-h group. No significant difference was found between 12-h group and 8-h group. AST, ALT, and LDH were significantly increased after preservation for 18-h than preservation for 8-h. No significant difference was found between 12-h group and 8-h group. The 7-d survival rate of animals in SWH group was higher than that in UW group. The change tendency of ATP, TAN, and EC was the same as the tendency of 7-d survival rate of animals. Conclusion These results suggest that levels of ATP, ADP, and AMP in hepatic tissues are closely associated with the survival rate, and SWH solution is effective in protecting hepatic energy metabolism.