Studies of murine malaria antigens using monoclonal antibodies |
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| Authors: | G Holmquist P Borwell A R Cattan H Wigzell |
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| Institution: | (1) Department of Immunology, University of Stockholm, S-10691 Stockholm, Sweden;(2) Department of Immunology, University of Uppsala, S-75123 Uppsala, Sweden;(3) Department of Immunology, Karolinska Institutet, S-10401 Stockholm, Sweden |
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| Abstract: | A panel of ten monoclonal antibodies made againstPlasmodium chabaudi andPlasmodium yoelii infected mouse erythrocytes were used for characterization of antigens present in murine malaria. Screening of the antibodies in ELISA with different fractions of infected erythrocytes revealed both species-specific and fraction-specific monoclonal antibodies (MAbs), but also MAbs cross-reacting between the species. Two MAbs bound normal erythrocyte components. Subcellular localization of the target antigens was studied by immunofluorescence and their molecular identity by immunoblotting after SDS-PAGE. Of the MAbs toP. yoelii, one reacted with a cytoplasmic granule component of 137 k and two others reacted with vacuole-associated antigens of 26 k and 25/70/73 k, respectively. The latter antibodies cross-reacted withP. chabaudi antigens. Of the MAbs toP. chabaudi, all were species specific, one reacting with parasite surface antigens of 79 and 250 k and two with a vacuole-associated antigen of 70 k. |
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