负压封闭引流装置用于全厚皮移植术后加压固定

目的 探讨封闭负压引流(vacuum sealing drainage,VSD)装置用于全厚皮移植术后加压固定对全厚皮片移植成活的影响.方法 新西兰大白兔24只,在脊柱两侧2个术区建立全厚皮片移植模型.每只兔2个术区分别采用VSD(A组)、传统缝合打包加压包扎(B组),做自身对照.第3、5、7天各随机处死8只兔,观察皮...

Experiment Study on Influence of Vacuum Sealing Drainage on Compression Bandage of Full-thickness Skin Grafting

Objective To explore the influence of vacuum sealing drainage （VSD） on compression bandage of full-thickness skin grafting. Methods A model of full-thickness skin grafting in 2 operation zones was established in the two sides of spinal column of 24 New Zealand white rabbits. The wounds were deal with by VSD （group A） and conventional suture and compression bandage （group B） randomly. About 3, 5, and 7 days after operation, the assessment of the center and edge area （suture area） of skin grafting was performed by a single observer blinded to the randomization. The survival rate was calculated. Samples were selected from skin grafting and observed under a light microscope after HE staining. The expression of vascular endothelial growth factor （VEGF） was detected by immunohistochemistry method. Results The assessment of the edge area of skin grafting in group A was significantly better than that in group B （P〈0. 05） ; the difference in the center area was not significant （P〉0. 05）. HE staining showed that in the edge area of skin grafting, the cell layer and structure were better in group A than that in group B, the difference in the center area was not significant （P〉0. 05）. Immunohistochemistry method showed that in the edge area of skin grafting, integrated optical density of VEGF was significantly higher in group A than in group B （P〈 0. 05）, it was not significantly in the center area （P〉0.05）. Conclusions VSD utilized on compression bandage of full-thickness skin grafting can significantly improve the qualitative appearance of grafting, promote wound healing and vascularization. VSD may possibly reduce scar hyperplasia.