血栓性血小板减少性紫癜患者血管性血友病因子前肽水平的研究

目的探讨血栓性血小板减少性紫癜（thrombotic thrombocytopenic purpura,TTP）患者血管内皮损伤程度,以及不同类型TTP之间血管内皮损伤差异性。方法纳入2005年4月2010年12月特发性TTP患者17例（A组）,继发性TTP患者15例（B组）,骨髓移植相关TTP患者2例（C组）,疑似TTP患者11例（D组）,共45例;另选取健康体检志愿者为对照组10例（E组）。采用双夹心酶联免疫吸附试验测定血管性血友病因子前肽（vonWillebrand factor propeptide,vWFpp）水平。结果 vWFpp水平为其与正常混合血浆的比值,A组为2.2,B组为2.34,C组为2.795,D组为1.72,E组为1.08。A、B、C、D组患者vWFpp水平与E组比较,差异有统计学意义（P〈0.05）,A、B、C、D组间比较,差异无统计学意义（P〉0.05）。结论 TTP患者vWFpp水平明显增高,提示血管内皮损伤明显,但vWFpp水平不能用于鉴别TTP类型。

Plasma von Willebrand Factor Propeptide Level in Patients with Thrombotic Thrombocytopenic Purpura

Objective To explore the severity of endothelium injury in patients with thrombotic thrombocytopenic purpura（TTP） and the differences among different subtypes of TTP.Methods The clinical data of 45 patients with TTP diagnosed between April 2005 and December 2010 were retrospectively analyzed.von Willebrand factor propeptide（vWFpp） was measured by sandwich ELISA in 17 patients with idiopathic TTP（group A）,15 patients with secondary TTP（group B）,2 patients with transplantation associated TTP（group C）,11 patients with suspected TTP（group D） and 10 control healthy volunteers（group E）.Results Median times of vWFpp of the five groups were 2.2,2.34,2.795,1.72,and 1.08 respectively.Plasma vWFpp levels of the first four groups didn′t differ much between each other（P0.05）,but the differences were significant compared with the data in the control group（P0.05）.Conclusions Significantly increased vWFpp level in patients with TTP indicates obvious endothelium injury.Nevertheless,it could not be used to differentiate TTP types.