| L-苏氨酸产生菌选育的研究 |
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| 引用本文： | 常尊学,李福德. L-苏氨酸产生菌选育的研究[J]. 沈阳药科大学学报, 1990, 0(3) |
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| 作者姓名： | 常尊学 李福德 |
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| 作者单位： | 沈阳药学院微生物教研室(常尊学)，沈阳药学院微生物教研室(李福德) |
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| 摘 要： | 用亚硝基胍(NTG)和紫外线(UV)诱变处理出发菌株T_(6-13),定向筛选α-氨基β-羟基戊酸(AHV)和S-(2-氯基乙基)-L-半胱氨酸(AEC)抗性突变株和蛋氨酸缺陷型,得一苏氨酸产生菌M_(8-31)(AHV~rAEC~rMet~-),可积累L-苏氨酸6.7mg/ml。再以M_(8-31)为出发菌株,进一步提高抗性和筛选α-氨基-4-乙硫丁酸(Eth)敏感突变株,获得一苏氨酸高产菌株ME7(AHV~rAEC~rMet~-Eth~s),在含有葡萄糖10%的培养基中发酵48h可积累L-苏氨酸17.5mg/ml。
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| 关 键 词： | 苏氨酸 诱变育种 缺陷型突变株 结构类似物抗性突变株 |
A STUDY ON THE BREEDING OF L-THREONINE PRODUCERS |
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| Chang Zunxue,Li Fude,Shenyang College of pharmacy. A STUDY ON THE BREEDING OF L-THREONINE PRODUCERS[J]. Journal of Shenyang Pharmaceutical University, 1990, 0(3) |
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| Authors: | Chang Zunxue Li Fude Shenyang College of pharmacy |
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| Abstract: | Brevibacterium flavum Te_(6-13) a glutamate producer, was treated with N-methyl-N'-nitro-N-nitrosoguanidine(NTG) and ultra-violet(UV) rays. As a result, a meth-ionine auxotrophic mutant M_(8-31) was derived which was resistant to both a-amino-β-h drox valeric acid(AHV) and S-(2-aminoeth 1)-L-cysteine (AEC). It accumu-lated 6.7mg/ml of L-threonine. With M_(8-31), as a starting strain, after receiving mi-xture mutational treatment with UV+LiC1 and NTG,a 2-amino-d-eth Isulphobutanoicacid(Eth) sensitive mutant ME_7 was obtained. It produced 17.5mg/ml of L-threoninein a medium containing 10% glucose in 48 hours at 33℃. |
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| Keywords: | Threonine Mutation breeding Auxotrophie mutant Analogue resistant mutant |
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