杭白菊指纹图谱研究及不同产地杭白菊质量比较

目的通过建立杭白菊HPLC指纹图谱,比较不同产地杭白菊成分的差异,为杭白菊的质量评价提供依据。方法采用Agilent SB-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.2%乙酸溶液为流动相梯...展开更多 目的通过建立杭白菊HPLC指纹图谱,比较不同产地杭白菊成分的差异,为杭白菊的质量评价提供依据。方法采用Agilent SB-C18色谱柱(250 mm×4.6 mm,5μm),以乙腈-0.2%乙酸溶液为流动相梯度洗脱,检测波长为348 nm,柱温30℃,流速1.0 mL/min,并对精密度、重复性、中间精密度、溶液稳定性进行考察。结果以绿原酸、木犀草苷、3,5-O-二咖啡酰基奎宁酸为参照峰建立了杭白菊指纹图谱共有模式,标定14个共有峰,精密度、重复性、稳定性良好。不同产地杭白菊指纹图谱相似度比较结果显示,除江苏盐城样品外,其他产地杭白菊质量相对稳定。结论该方法准确、重复性好,可为杭白菊的内在质量评价提供依据。收起 Objective To establish the fingerprints of Hangzhou white Chrysanthemi Flos by HPLC;To compare the differences in the compositions of Hangzhou white Chrysanthemi Flos from different producing areas;To provide references for quality evaluation of Hangzhou white Chrysanth...MORE Objective To establish the fingerprints of Hangzhou white Chrysanthemi Flos by HPLC;To compare the differences in the compositions of Hangzhou white Chrysanthemi Flos from different producing areas;To provide references for quality evaluation of Hangzhou white Chrysanthemi Flos.Methods The chromatographic separation was performed on an Agilent SB-C18 column (250mm×4.6mm,5μm).The mobile phase was acetonitrile-0.2% acetic acid aqueous solution in gradient elution.The detection wavelength was 348nm;the column temperature was 30℃;the flow rate was 1.0mL/min.The precision,repeatability,intermediate precision and solution stability were investigated.Results The common pattern of fingerprints of Hangzhou white Chrysanthemi Flos was established by using chlorogenic acid,luteolin and 3,5-O-dicaffeoylquinic acid as reference peaks.14 common peaks were marked. The precision,repeatability and stablility were good.Similarity comparison of fingerprints of Hangzhou white Chrysanthemi Flos from different producing areas showed that the quality of Hangzhou white Chrysanthemi Flos was relatively stable except for Yancheng (Jiangsu Province).Conclusion The method is accurate and reproducible,and provide reference for the internal quality evaluation of Hangzhou white Chrysanthemi Flos.FEWER

作者 徐洪明 金辉辉 王昕佳 王伟 王如伟 姚建标

Study on Fingerprints of Hangzhou White Chrysanthemi Flos and Comparison of Hangzhou White Chrysanthemi Flos from Different Producing Areas