基于iTRAQ的定量蛋白质组学技术探索淡色库蚊越冬机制

目的　比较淡色库蚊越冬后与滞育准备阶段蛋白表达差异，探索淡色库蚊越冬休眠（滞育）的机制。方法　采用同位素标记相对和绝对定量（iTRAQ）技术，对越冬滞育及准备阶段淡色库蚊进行定量蛋白质组学分析。结果　淡色库蚊越冬滞育前后共鉴定出244种差异表达蛋白，其中上调蛋白126种、下调蛋白118种。生物信息学分析表明，这些差异表达蛋白与能量产生及转化、脂代谢、细胞骨架重塑、糖代谢、蛋白质转运、分子伴侣、应激耐受以及各种代谢酶等有关。结论　本研究首次采用现代蛋白质组学工具鉴定淡色库蚊越冬滞育相关蛋白，初步揭示了与淡色库蚊越冬滞育相关的KEGG通路及蛋白，进一步扩展了对蚊媒越冬滞育机制的理解。

Study on the mechanisms underlying overwintering of Culex pipiens pallens using iTRAQ-based quantitative proteomic analysis

Objective　To compare the difference of protein expression between the post?overwintering stage and the diapauses preparation stage in Culex pipiens pallens, so as to reveal the mechanisms underlying the overwintering diapause of Cx. pipiens pallens. Methods　A quantitative proteomic analysis was performed in Cx. pipiens pallens before and after overwintering diapause by using isobaric tags for relative and absolute quantification (iTRAQ) labeling. Results　A total of 244 differentially expressed proteins were identified in Cx. pipiens pallens before and after overwintering diapause, including 126 up?regulated proteins and 118 down?regulated proteins. iTRAQ?based quantitative proteomic analysis revealed that these differentially expressed proteins were linked to function and energy production and conversion, lipid metabolism, remodeling of cytoskeleton, carbohydrate metabolism, protein transport, molecular chaperones, stress tolerance and metabolic enzymes. Conclusions　This is the first study to identify the overwintering diapause?related proteins in Cx. pipiens pallens using proteomics tools, which reveals KEGG pathways and GO terms associated with the overwintering diapauses of Cx. pipiens pallens. Our findings provide additional understandings pertaining to the mechanisms underlying the overwintering diapauses of Cx. pipiens pallens.