| HPLC法测定田参正脉含片中二氢杨梅素的含量 |
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| 引用本文： | 冯炜业,王立兴. HPLC法测定田参正脉含片中二氢杨梅素的含量[J]. 海峡药学, 2010, 22(1): 47-49 |
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| 作者姓名： | 冯炜业 王立兴 |
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| 作者单位： | 1. 福建医科大学附属第一医院药学部,福州,350004
2. 福州长富星新药开发有限公司,福州,350002 |
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| 摘 要： | 目的建立人参含片中二氢杨梅素的含量测定方法。方法采用高效液相色谱法，色谱柱为Lichrospher C18柱（4．6×250mm，5μm）。ODS预柱，流动相为甲醇0．1％磷酸（25：75）；检测波长290nm；流速为1．0mL·min^-1。结果本法精密度高，稳定性好。二氢杨梅素溶液在0．00532～0．1064mg·mL^-1的浓度范围内具有良好的线性关系（r=0．9997），平均加样回收率为101．62％，RSD为1．55％（n=6）。结论本方法简便，准确，重现性好，可作为该制剂的含量测定方法。
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| 关 键 词： | 田参正脉含片 二氢杨梅素 高效液相色谱法 |
Determination of dihydromyricetine in renshen buccal tablet by HPLO |
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| FENG Wei-ye,WANG Li-Xing. Determination of dihydromyricetine in renshen buccal tablet by HPLO[J]. Strait Pharmaceutical Journal, 2010, 22(1): 47-49 |
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| Authors: | FENG Wei-ye WANG Li-Xing |
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| Affiliation: | 1.The Department of Pharmacy of The First Affiliated Hospital of Fujian Medical University;Fuzhou 350004;China;2.Fuzhou Changfuxing Pharmaceutical Development Co.;Ltd.;Fuzhou 350002;China |
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| Abstract: | OBJECTIVE To establish a method for determination of Dihydromyricetine in Renshen buccal tablet,METHODS Using a HPLC method with Lichrospher C18（4.6 × 250mm, 5μm）column, ODS pre-column; the mobile phase was methanol -0.1 phosphoric acid （25：75）;the detection wavelength was set at 290nm; flow rate was 1.0mL· min^-1. The precision and stability were fine.the linean range within 0.00532- 0. 1064mg· mL^-1,r = 0.9997.The average rocoveries of dihydromyricetine（n = 6） was 101.62% with RSD 1.55% .CONCLUSION The method was simple, accurate and reproducible, and can be used to determine the dihydromyricetine in Tianshen Zhengmai buccal tablet. |
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| Keywords: | Tianshen Zhengmai buccal tablet Dihydromyricetine HPLC |
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