精子洗涤对精液中HBV DNA含量的影响

目的：检查HBV感染者精液是否存在乙型肝炎病毒（HBV），探讨精子洗涤是否能去除其中的HBV DNA。方法：用实时荧光定量PCR（FQ-PCR）法检测57名血清HBsAg阳性患者精液中HBV DNA，采用Percoll非连续梯度离心及上游法分离其中21份精液，并对洗涤后标本进行HBV DNA检测。结果：62份精液标本中有15份HBV DNA阳性，阳性率为24.19%;21份用于洗涤的精液标本中有，有7份HBV DNA阳性，洗涤后仍有6份标本阳性。结论：部分HBV感染者精液具有传染性，精子洗涤不能完全去除精液中的乙肝病毒。将HBV感染者的精液用于人工授精或体外受精应慎重。

The effect of sperm washing on hepatitis B virus concentration in semen

Objective: To detect hepatitis B virus (HBV) in semen and to find if sperm washing removes HBV DNA. Methods: Amplification by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect viral DNA in semen samples of 57 HBsAg-seropositive patients.Twenty-one semen samples were washed,and spermatozoa were separated by discontinuous Percoll gradient centrifugation and the `swim-up' method.The HBV DNA in post-washing samples was measured by FQ-PCR. Results: HBV DNA was detected in 15 of 62 semen samples,the positive rate was 24.19%.There were 7 positive cases in 21 samples before washing,the 6 cases except one were positive after washing. Conclusion: Part of semen of HBV infected patients is infective,and sperm washing does not remove HBV.It is cautious to use semen of the HBV infected patients in IVI or IVF.