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| 用间期细胞FISH技术快速分析性腺发育不全患者的性染色体数目异常 | |
| 引用本文： | 刘青杰,封江彬,陆雪,王晓维,刘蕴奡,马芬,姚素艳,沈岩. 用间期细胞FISH技术快速分析性腺发育不全患者的性染色体数目异常[J]. 中国优生与遗传杂志, 2004, 12(1): 32-33,F003 |
| 作者姓名： | 刘青杰 封江彬 陆雪 王晓维 刘蕴奡 马芬 姚素艳 沈岩 |
| 作者单位： | 1. 国家人类基因组北方研究中心,100176;中国疾病预防控制中心辐射防护与核安全医学所,100088 2. 中国疾病预防控制中心辐射防护与核安全医学所,100088 3. 国家人类基因组北方研究中心,100176 |
| 基金项目： | 教育部留学回国人员科研启动基金，国家高技术研究发展计划(863计划) |
| 摘 要： | 目的探索用间期细胞FISH快速分析男性性腺发育不全患者染色体数目异常的方法.方法应用生物素和地高辛分别标记X染色体特异性人工细菌染色体(BAC)DNA以及Yq12区域重复序稽pY3.4 DNA,对15例男性性腺发育不全患者外周血淋巴细胞间期核进行双色荧光原位杂交(FISH)分析.结果淋巴细胞间期核FISH和常规染色体分析结果一致,可发现47,XXY、46,XX和46,XY/47,XXY嵌合体等.结论间期双色FISH可以快速分析男性性腺发育不全患者的性染色体数目异常,为临床诊断提供依据. |
| 关 键 词： | 间期荧光原位杂交 性腺发育不全 染色体数目异常 |
| 文章编号： | 1006-9534(2004)01-0032-02 |
Rapid detection of numerical sex chromosome abnormality on male gonad agenesis by interphase FISH |
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| LIU Qing-jie,Feng Jiang-bin,LU Xue,WANG Xiao-wei,YAO Su-yan,et al.. Rapid detection of numerical sex chromosome abnormality on male gonad agenesis by interphase FISH[J]. Chinese Journal of Birth Health & Heredity, 2004, 12(1): 32-33,F003 | |
| Authors: | LIU Qing-jie Feng Jiang-bin LU Xue WANG Xiao-wei YAO Su-yan et al. |
| Abstract: | Objective: To explore the interphase fluorescence in situ hybridization (FISH) method to rapidly detect the numerical sex chromosome abnormality of gonad agenesis patients. Methods: X specific BAC (bacterial artificial chromosome) DNA and pY3.4 DNA were labeled with biotin-14-dATP or digoxigenin-11dUTP, respectively. Two colors interphase FISH was used to analyze the lymphocytes of gonad agenesis patients. Results: The result of interphase FISH method on gonad agenesis was in good agreement with that of traditional chromosome analysis, no matter 47,XXY?46,XY/47,XXY or sex reverse. Conclusion: The two colors interphase FISH method could be used to rapidly detect the numerical sex chromosome abnormality of gonad agenesis. |
| Keywords: | Interphase FISH Gonad agenesis Numerical chromosome abnormality |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |