Knockdown of RCAN1.4 Increases Susceptibility to FAS-mediated and DNA-damage-induced Apoptosis by Upregulation of p53 Expression |
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| Authors: | Young Sun Kim Hong Joon Lee Chorong Jang Ho-Shik Kim Young-Jin Cho |
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| Affiliation: | 1Department of Pharmacology, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.;2Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea.;3Cell Death Disease Research Center, College of Medicine, The Catholic University of Korea, Seoul 137-701, Korea. |
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| Abstract: | Despite the potential importance of the human regulator of calcineurin 1 (RCAN-1) gene in the modulation of cell survival under stress, little is known about its role in death-inducing signal pathways. In this study, we addressed the effects of RCAN1.4 knockdown on cellular susceptibility to apoptosis and the activation of death pathway proteins. Transfection of siRNAs against RCAN1.4 resulted in enhanced Fas- and etoposide-induced apoptosis, which was associated with increased expression and translocation of Bax to mitochondria. Our results suggest that enhanced expression and activation of p53 was responsible for the upregulation of Bax and the increased sensitivity to apoptosis, which could be reversed by p53 knockdown. To explain the observed upregulation of p53, we propose a downregulation of the ubiquitin ligase HDM2, probably translationally. These findings show the importance of appropriate RCAN1.4 expression in the modulation of cell survival and reveal a link between RCAN1.4 and p53. |
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| Keywords: | RCAN1 Apoptosis p53 Bax HDM2 |
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