LICC对H＿（22）腹水型肝癌的抑制作用及对荷瘤鼠细胞免疫的影响

应用正交试验对影响ＬＩＣＣ的生成及其杀伤较大的因素（诱导时间，ＩＬ－２浓度，细胞毒细胞分化因子－ＣＣＤＦ，效应细胞与靶细胞的比例）进行筛选，以获得高杀伤活性的ＬＩＣＣ。对荷瘤动物分组用毛细管法进行白细胞移动抑制试验（ＭＩＴ），在Ｈ＿２２攻击后的第５周、第７周ＬＩＣＣ实验动物的ＭＩＴ阳性率明显高于对照组，其差异有显著性（Ｐ＜０．０５），提示ＬＩＣＣ能维持或提高机体对Ｈ＿２２的特异性细胞免疫功能。同时，实验组动物死亡率明显低于对照组，荷瘤存活时间较对照组也明显延长，提示ＬＩＣＣ对荷瘤动物有保护作用。是继ＬＡＫ细胞、ＴＩＬ细胞之后的又一种新型的肿瘤过继性免疫治疗细胞。

EFFECT OF LICC ON INHIBITION OF H_22 HEPATIMA CELL ANDCYTOIMUNITY OF TUMOR LOADED RATS

A series of experimental studies on the effects of LICC to the antitumor activity and cytoim-munity of the tumor loaded animal are reported.The factors affecting the genesis of LICC andits activity,such as induction time,concentration of IL-2, cytotoxic cell defferentiation factor(CCDF)and ratio of effector cell and target cell were screened by orthogonal test.Thus the opti-mal condition to attain the highest activity of LICC was obtained. Leucocyte migration inhibitingtests were performed in tumor loaded animal at the 5th and 7th weeks after the inplantation ofH_22 cells. In the LICC group,the positivity of MIT was significantly higher than that of the con-trols with P<0.005.The stivival time of the former was longer than that of the latter. The re-sults reveal that LICC has the protective effect on the tumor loaded animal and it is a new cellfor the adoptive immunotherapy of tumor after the discovery of LAK and TIC.